ENTPD3 Human, sf9 Bioactive

ENTPD3 is a protein-coding gene that contains four apyrase-conserved regions, characteristic of NTPDases . The human recombinant form of ENTPD3 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 451 amino acids, with a molecular mass of approximately 50.7 kDa . This recombinant protein is expressed with a six-amino-acid His tag at the C-terminus and is purified using proprietary chromatographic techniques .

ENTPD3 exhibits a threefold preference for the hydrolysis of ATP over ADP, similar to other E-type nucleotidases . The enzyme’s specific activity is greater than 250,000 pmol/min/µg, defined as the amount of enzyme that hydrolyzes ATP per minute at pH 7.5 and 37°C . This high level of activity makes ENTPD3 a crucial player in regulating extracellular nucleotide levels, which can influence various physiological processes, including inflammation, immunity, and cancer .

The ENTPD3 protein solution is formulated in phosphate-buffered saline (pH 7.4) with 10% glycerol . For short-term storage, it is recommended to keep the solution at 4°C if it will be used within 2-4 weeks. For long-term storage, the solution should be frozen at -20°C, and it is advisable to add a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) to prevent degradation . It is important to avoid multiple freeze-thaw cycles to maintain the protein’s integrity .

ENTPD3 has significant applications in research, particularly in studies related to nucleotide metabolism and signaling. Its role in hydrolyzing extracellular nucleotides makes it a valuable tool for investigating the mechanisms underlying various physiological and pathological processes . Additionally, the recombinant form of ENTPD3 is used in laboratory research to explore its potential therapeutic applications in conditions such as inflammation and cancer .