Enterokinase Porcine
Description
If a fusion tag is located in the N-terminus with an enterokinase site, enterokinase will be able to remove the fusion tag and to generate the protein exactly as you need without adding any unwanted residues. ProSpec’s enterokinase is a highly purified enterokinase from porcine. The enzyme has been extensively purified and tested to ensure that there are no other contaminating proteases.
Product Specs
Product Science Overview
Enteropeptidase was first discovered by Ivan Pavlov, a renowned physiologist who was awarded the Nobel Prize in Physiology or Medicine in 1904 for his studies on gastrointestinal physiology . Pavlov’s student, N. P. Schepowalnikov, demonstrated that duodenal secretions could activate pancreatic proteolytic enzymes, leading to the identification of enteropeptidase as a key activator of these enzymes .
Enteropeptidase is a type II transmembrane serine protease localized to the brush border of the duodenal and jejunal mucosa . It is synthesized as a zymogen (proenteropeptidase) that requires activation by another protease, such as trypsin or duodenase . The enzyme consists of a disulfide-linked heavy chain (82-140 kDa) that anchors it to the intestinal brush border membrane and a light chain (35-62 kDa) that contains the catalytic subunit .
The primary function of enteropeptidase is to convert trypsinogen, an inactive zymogen, into its active form, trypsin . This activation is crucial because trypsin, in turn, activates other pancreatic digestive enzymes, facilitating the breakdown of proteins in the small intestine .
Enteropeptidase is essential for proper digestion and nutrient absorption. In the absence of this enzyme, the activation of trypsinogen is impaired, leading to significant digestive issues . The enzyme’s activity ensures that digestive enzymes are activated only in the intestine, preventing premature activation that could damage the pancreas and other tissues .
Extensive research has been conducted on enteropeptidase, focusing on its structure, function, and role in various physiological processes . Studies have also explored the enzyme’s involvement in certain disorders and potential inhibitors that could modulate its activity . Understanding enteropeptidase’s mechanisms and interactions can provide insights into developing therapeutic interventions for digestive disorders and other related conditions .
