ENO3 Human

Enolase-3 Human Recombinant
Cat. No.
BT17033
Source
E.coli.
Synonyms
Enolase 3 (beta, muscle), Muscle-specific enolase, Skeletal muscle enolase, MSE, 2-phospho-D-glycerate hydrolyase, beta-enolase, GSD13, EC 4.2.1.11, EC 4.2.1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ENO3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 454 amino acids (1-434) and having a molecular mass of 49.0 kDa.
ENO3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
ENO3, one of three enolase isoenzymes found in mammals, forms a homodimer and plays a crucial role in skeletal muscle cells of adults. It participates in the glycolytic pathway by converting phosphoglyceric acid to phosphenolpyruvic acid. Genetic mutations affecting the ENO3 gene can lead to metabolic myopathies, often characterized by reduced enzyme stability.
Description
Recombinantly produced in E.coli, ENO3 Human is a single, non-glycosylated polypeptide chain comprising 454 amino acids (specifically, residues 1-434). With a molecular weight of 49.0 kDa, it features a 20 amino acid His-tag at the N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The ENO3 solution is provided at a concentration of 0.5mg/ml and is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 1mM DTT, 0.1M NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the ENO3 solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein (either 0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of ENO3 is determined by SDS-PAGE analysis and is consistently greater than 95%.
Specific Activity
The specific activity of ENO3 is measured to be greater than 1.5 units per milliliter.
Unit Definition
One unit of ENO3 activity is defined as the amount of enzyme required to catalyze the conversion of 1.0 micromole of 2-phosphoglycerate to phospho(enol)pyruvate per minute at a pH of 7.5 and a temperature of 25°C.
Synonyms
Enolase 3 (beta, muscle), Muscle-specific enolase, Skeletal muscle enolase, MSE, 2-phospho-D-glycerate hydrolyase, beta-enolase, GSD13, EC 4.2.1.11, EC 4.2.1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAMQKIFARE ILDSRGNPTV EVDLHTAKGR FRAAVPSGAS TGIYEALELR DGDKGRYLGK GVLKAVENIN STLGPALLQK KLSVADQEKV DKFMIELDGT ENKSKFGANA ILGVSLAVCK AGAAEKGVPL YRHIADLAGN PDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGASSFKE AMRIGAEVYH HLKGVIKAKY GKDATNVGDE GGFAPNILEN NEALELLKTA IQAAGYPDKV VIGMDVAASE FYRNGKYDLD FKSPDDPARH ITGEKLGELY KSFIKNYPVV SIEDPFDQDD WATWTSFLSG VNIQIVGDDL TVTNPKRIAQ AVEKKACNCL LLKVNQIGSV TESIQACKLA QSNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEALGDK AIFAGRKFRN PKAK.

Product Science Overview

Structure and Function

Enolase-3 is a homodimeric enzyme predominantly found in skeletal muscle cells of adults. It plays a crucial role in the glycolytic pathway, specifically in the conversion of 2-phosphoglycerate to phosphoenolpyruvate . This step is essential for the production of ATP, which provides energy for various cellular processes.

Gene Expression and Regulation

The ENO3 gene spans approximately 6 kilobases and contains 12 exons. The first exon is non-coding, and the gene’s regulatory region includes several muscle-specific regulatory elements. These elements ensure that ENO3 is highly expressed in muscle tissues, where it is necessary for muscle development and regeneration .

Isoform Switching

During muscle development in rodents, there is a switch from alpha-enolase to beta-enolase. This switch is crucial for the proper functioning of muscle tissues. In humans, beta-enolase continues to play a significant role in muscle maintenance and repair throughout adulthood .

Clinical Significance

Mutations in the ENO3 gene have been associated with metabolic myopathies, a group of disorders characterized by muscle weakness and exercise intolerance. These conditions are often linked to the low stability of the enzyme, which impairs its function in the glycolytic pathway .

Recombinant Enolase-3

Human recombinant Enolase-3 is produced using recombinant DNA technology, which involves inserting the ENO3 gene into a suitable expression system, such as bacteria or yeast. This allows for the large-scale production of the enzyme, which can be used for various research and therapeutic purposes.

Multifunctional Roles

Beyond its primary role in glycolysis, Enolase-3 has been found to have several other functions. It acts as a DNA-binding protein and a tubulin/microtubule-binding protein during myogenesis. Additionally, it has been implicated in hypoxia tolerance, tumor suppression, and cell surface plasminogen binding .

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