Endoglin Human

Endoglin Human Recombinant
Cat. No.
BT21737
Source
Escherichia Coli.
Synonyms
CD105, ENG, END, ORW, HHT1, ORW1, FLJ41744, Endoglin.
Appearance
Sterile Filtered colorless liquid formulation.
Purity
Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

Endoglin Human Recombinant extracellular domain produced in E.Coli is a single, glycosylated, Polypeptide containing 151 amino acids (26-176) and having a molecular mass of 43 kDa.
The Endoglin is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Endoglin, a type I membrane glycoprotein found on cell surfaces, is an integral part of the TGF beta receptor complex. This protein exists as a homodimer with a molecular weight of 180 kDa, held together by disulfide bonds. Endoglin is primarily located on endothelial cells but is also present on activated macrophages, fibroblasts, and smooth muscle cells. As a component of the TGF-beta1 receptor complex, Endoglin is believed to be involved in binding various growth factors including TGF-beta1, TGF-beta3, activin-A, BMP-2, and BMP-7. Beyond its role in TGF-beta signaling, Endoglin is thought to contribute to cytoskeletal organization, influencing cell shape and movement. It plays a crucial role in cardiovascular development and vascular remodeling, with its expression being regulated throughout heart development. Notably, mice lacking the endoglin gene experience cardiovascular abnormalities and do not survive.
Description
This product consists of the extracellular domain of human Endoglin, recombinantly produced in E.Coli. It is a single, glycosylated polypeptide chain comprising 151 amino acids (residues 26-176), resulting in a molecular weight of 43 kDa. The purification process involves proprietary chromatographic methods.
Physical Appearance
The product is a clear, colorless liquid solution that has been sterilized by filtration.
Formulation
The Endoglin protein is supplied in a solution containing 50mM Tris-Acetate (pH 7.5), 1mM EDTA, and 20% Glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (either 0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of this product exceeds 90%, as determined by two methods: Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE).
Synonyms
CD105, ENG, END, ORW, HHT1, ORW1, FLJ41744, Endoglin.
Source
Escherichia Coli.

Product Science Overview

Structure and Function

Endoglin is a 90 kDa protein that forms a disulfide-linked homodimer on the cell surface . It associates with TGF-β type I receptors, including the endothelial cell-specific ALK1 (activin receptor-like kinase 1) and the ubiquitous ALK5, as well as the TGF-β type II receptor . This association is essential for the regulation of angiogenesis and vascular development.

Role in Disease

Mutations in the endoglin gene are responsible for Hereditary Hemorrhagic Telangiectasia (HHT) type 1, a vascular disorder characterized by defective angiogenesis, arteriovenous malformations, telangiectasia, and frequent nosebleeds (epistaxis) . Additionally, endoglin is implicated in preeclampsia, a pregnancy-related condition characterized by high blood pressure and damage to other organ systems .

Recombinant Human Endoglin

Recombinant human endoglin is produced using a mouse myeloma cell line, NS0-derived human endoglin/CD105 protein . It is typically used in research to study its role in angiogenesis and related diseases. The recombinant protein is purified to a high degree, with a purity greater than 90% as determined by SDS-PAGE . It is also tested for endotoxin levels to ensure it is suitable for use in various biological assays .

Applications

Recombinant human endoglin is used in various research applications, including:

  • Studying TGF-β signaling pathways: As a co-receptor for TGF-β ligands, endoglin is crucial for understanding the signaling mechanisms involved in angiogenesis and vascular development.
  • Investigating vascular diseases: Research on endoglin helps in understanding the molecular basis of diseases like HHT and preeclampsia.
  • Drug development: Endoglin is a potential therapeutic target for diseases involving abnormal blood vessel formation.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

Endoglin (27-581) Mouse
Endoglin (27-581) Mouse Recombinant
Cat. No.
BT21639
Source
Sf9, Baculovirus cells.
Endoglin Human, His
Endoglin Human Recombinant, His-Tag
Cat. No.
BT21843
Source
Escherichia Coli.
Endoglin Human, Sf9
Endoglin Human Recombinant, Sf9
Cat. No.
BT21925
Source
Sf9 Insect Cells.
Endoglin Mouse
Endoglin Mouse Recombinant
Cat. No.
BT21993
Source
Insect Cells.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.