ENA 78 Rat

The rat recombinant version of CXCL5 is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain containing 93 amino acids. It has a molecular mass of approximately 10.0 kDa . The protein is purified using proprietary chromatographic techniques to ensure high purity .

CXCL5 plays a crucial role in the immune response by acting as a chemoattractant for neutrophils. It is involved in the recruitment and activation of neutrophils to sites of inflammation, thereby contributing to the body’s defense mechanisms against infections and injuries . The activity of CXCL5 is determined by its ability to chemoattract human peripheral blood neutrophils within a concentration range of 10.0-100.0 ng/ml .

The lyophilized form of ENA-78 is stable at room temperature for up to three weeks. However, for long-term storage, it is recommended to keep the protein desiccated below -18°C. Upon reconstitution, CXCL5 should be stored at 4°C for short-term use (2-7 days) and below -18°C for future use. To enhance stability during long-term storage, adding a carrier protein such as 0.1% Human Serum Albumin (HSA) or Bovine Serum Albumin (BSA) is advisable .

CXCL5 is widely used in research to study its role in inflammation and immune response. It is also utilized in various assays to investigate the mechanisms of neutrophil recruitment and activation. Understanding the function and regulation of CXCL5 can provide insights into the development of therapeutic strategies for inflammatory diseases and conditions involving neutrophil infiltration .

In summary, Epithelial Neutrophil-Activating Protein 78 (Rat Recombinant) (CXCL5) is a significant chemokine involved in the immune response, particularly in neutrophil activation and recruitment. Its recombinant form, produced in E. coli, is a valuable tool for research in immunology and inflammation.