EMAP II Human
Endothelial-Monocyte Activating Polypeptide II Human Recombinant
Cat. No.
BT30447
Source
Escherichia Coli.
Synonyms
AIMP1, EMAP2, EMAP-2, EMAPII, SCYE1, Multisynthetase complex auxiliary component p43, Endothelial monocyte-activating polypeptide 2, EMAP-II, p43.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
EMAP-II Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 166 amino acids and having a molecular mass of 18.3 kDa.
The EMAP-II is purified by proprietary chromatographic techniques.
The EMAP-II is purified by proprietary chromatographic techniques.
Product Specs
Introduction
EMAP-II, also known as SCYE1, is a cytokine produced by tumors that exhibits a wide range of effects on endothelial cells, monocytes, and neutrophils. It acts as an inhibitor of endothelial cell proliferation, vasculogenesis, and neovessel formation, and it can also trigger apoptosis. Additionally, EMAP-II exhibits chemotactic properties towards neutrophils and monocytes, and it stimulates myeloperoxidase activity in neutrophils. Clinically, EMAP-II holds promise in inhibiting angiogenesis in vascular beds and suppressing the growth of both primary and secondary tumors without affecting healthy tissues. SCYE1 is specifically produced during apoptosis and plays a role in regulating angiogenesis, inflammation, and wound healing. The release of SCYE1 increases the sensitivity of tumor-associated vasculature to tumor necrosis factor. Interestingly, the precursor protein of EMAP-II is identical to the p43 subunit, a component of the multi-tRNA synthetase complex, and it regulates the aminoacylation activity of tRNA synthetase in healthy cells. EMAP-2 contributes to the stimulation of inflammatory responses following proteolytic cleavage within tumor cells.
Description
Recombinant Human EMAP-II, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 166 amino acids. It has a molecular weight of 18.3 kDa. The purification of EMAP-II is achieved through proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Lyophilized from a concentrated (1mg/ml) solution in 20mM sodium Phosphate buffer pH 7.5 and 130mM sodium chloride.
Solubility
To reconstitute lyophilized EMAP-II, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. The resulting solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized EMAP-II remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated below -18°C. After reconstitution, EMAP-II should be stored at 4°C for 2-7 days. For extended storage, it should be kept at -18°C. Avoid repeated freeze-thaw cycles.
Purity
Greater than 98.0% as determined by (a) RP-HPLC analysis and (b) SDS-PAGE analysis.
Biological Activity
The biological activity of EMAP-II is determined by its apoptotic effect on MCF-7 cells. The effective concentration range for inducing apoptosis is 20-30 ng/ml.
Synonyms
AIMP1, EMAP2, EMAP-2, EMAPII, SCYE1, Multisynthetase complex auxiliary component p43, Endothelial monocyte-activating polypeptide 2, EMAP-II, p43.
Source
Escherichia Coli.
Amino Acid Sequence
SKPIDVSRLD LRIGCIITAR KHPDADSLYV EEVDVGEIAP RTVVSGLVNH VPLEQM QNRM VILLCNLKPA KMRGVLSQAM VMCASSPEKI EILAPPNGSV PGDRITFDAF PGEPDKELNP KKKIWEQIQP DLHTNDECVA TYKGVPFEVK GKGVCRAQTM SNSGIK.
Product Science Overview
Structure and Synthesis
EMAP-II is synthesized as the C-terminal portion of a biologically inactive precursor protein that contains a propeptide of 146 amino acid residues . The mature form of EMAP-II is an 18.3 kDa protein . Recombinant Human EMAP-II (rHuEMAP-II) is produced in Escherichia coli and is a single non-glycosylated polypeptide chain containing 166 amino acids .
Biological Functions
EMAP-II exerts several critical functions:
- Inhibition of Endothelial Cell Proliferation: It prevents the proliferation of endothelial cells, which is crucial for the formation of new blood vessels .
- Induction of Apoptosis: EMAP-II can induce programmed cell death in various cell types .
- Chemotactic Activity: It attracts neutrophils and monocytes to the site of action .
- Myeloperoxidase Activity: EMAP-II induces myeloperoxidase activity from neutrophils .
Clinical Significance
Research and Applications
Storage and Handling
Recombinant Human EMAP-II is typically supplied as a sterile filtered, white lyophilized powder. It is recommended to reconstitute it in sterile distilled water or an aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL . The product should be stored at -20°C to -70°C and should avoid repeated freeze-thaw cycles to maintain its stability .
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