AIMP1 Human
Aminoacyl tRNA Synthetase Complex-Interacting Multifunctional Protein 1 Human Recombinant
Cat. No.
BT30334
Source
Escherichia Coli.
Synonyms
Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, Multisynthase complex auxiliary component p43, AIMP1, EMAP2, SCYE1, p43, EMAPII.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
AIMP1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 356 amino acids (1-336 a.a.) and having a molecular mass of 39.2kDa (Molecular size on SDS-PAGE will appear higher). The AIMP1 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
AIMP1, also known as EMPA2 or p43, is a cytokine released during apoptosis. It plays a role in regulating angiogenesis (blood vessel formation), inflammation, and wound healing. AIMP1 can sensitize blood vessels associated with tumors, making them more susceptible to the effects of tumor necrosis factor (TNF). Additionally, when broken down within tumor cells, AIMP1 can trigger inflammatory responses.
Description
This product consists of a recombinant human AIMP1 protein that has been engineered to include a 20 amino acid His tag at its N-terminus. It is produced in E. coli and exists as a single, non-glycosylated polypeptide chain comprising 356 amino acids (specifically, amino acids 1-336 of the AIMP1 sequence). The molecular weight of the protein is 39.2 kDa, although it might appear larger on SDS-PAGE. The purification of AIMP1 is achieved using proprietary chromatographic methods.
Physical Appearance
A clear and colorless solution that has been sterilized by filtration.
Formulation
The AIMP1 protein is provided at a concentration of 0.25 mg/ml in a buffer solution containing 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 2 mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein like 0.1% HSA or BSA is suggested for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of this product is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, Multisynthase complex auxiliary component p43, AIMP1, EMAP2, SCYE1, p43, EMAPII.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MLPAVAVSEP VVLRFMIFCR LLAKMANNDA VLKRLEQKGA EADQIIEYLK QQVSLLKEKA ILQATLREEK KLRVENAKLK KEIEELKQEL IQAEIQNGVK QIPFPSGTPL HANSMVSENV IQSTAVTTVS SGTKEQIKGG TGDEKKAKEK IEKKGEKKEK KQQSIAGSAD SKPIDVSRLD LRIGCIITAR KHPDADSLYV EEVDVGEIAP RTVVSGLVNH VPLEQMQNRM VILLCNLKPA KMRGVLSQAM VMCASSPEKI EILAPPNGSV PGDRITFDAF PGEPDKELNP KKKIWEQIQP DLHTNDECVA TYKGVPFEVK GKGVCRAQTM SNSGIK.
Product Science Overview
Structure and Function
AIMP1 is a polypeptide with both cytokine and tRNA-binding activities. It is associated with several aminoacyl-tRNA synthetases within the multisynthetase complex. The protein has a tRNA-binding domain that, upon cleavage from the p43 component of the multisynthetase complex, becomes an independent domain with inflammatory cytokine activity .
The primary functions of AIMP1 include:
- Stimulation of Catalytic Activity: AIMP1 stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase .
- Inflammatory Cytokine Activity: It possesses inflammatory cytokine activity, which is crucial in the activation of endothelial cells and the chemotaxis of neutrophils and mononuclear phagocytes .
- Angiogenesis and Wound Healing: AIMP1 is involved in the control of angiogenesis, inflammation, and wound healing. It is specifically induced by apoptosis and plays a role in rendering tumor-associated vasculature sensitive to tumor necrosis factor .
Gene and Protein Expression
The AIMP1 gene is located on chromosome 4q24 and encodes a 312-amino acid polypeptide. The predicted 34-kD precursor is cleaved to produce an active 20-kD product . Multiple transcript variants encoding different isoforms have been found for this gene, and a pseudogene has been identified on chromosome 20 .
Biological Significance
Clinical Relevance
Diseases associated with AIMP1 include Leukodystrophy, Hypomyelinating, 3 and Autosomal Recessive Non-Syndromic Intellectual Disability . The protein’s role in inflammation and angiogenesis also makes it a potential target for therapeutic interventions in various inflammatory and cancer-related conditions.
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