ELP4 Human

Elongator Acetyltransferase Complex Subunit 4 Human Recombinant
Cat. No.
BT1208
Source
Escherichia Coli.
Synonyms
Elongator complex protein 4, ELP4, Elongator Acetyltransferase Complex Subunit 4, hELP4, PAX6 neighbor gene protein, C11orf19, PAXNEB, dJ68P15A.1, PAX6NEB.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ELP4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 447 amino acids (1-424 a.a.) and having a molecular mass of 49kDa.
ELP4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Elongator Acetyltransferase Complex Subunit 4, also known as ELP4, is a protein-coding gene. It is a subunit of the RNA polymerase II elongator complex. ELP4 complements phenotypes of yeast ELP4 deletion mutants and is linked to Rolandic epilepsy.
Description
Recombinant human ELP4 protein, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 447 amino acids (1-424 a.a.). It has a molecular mass of 49 kDa. ELP4 is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile filtered solution.
Formulation
ELP4 protein solution (1 mg/ml) in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For long-term storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freezing and thawing.
Purity
Purity greater than 80.0% as determined by SDS-PAGE.
Synonyms
Elongator complex protein 4, ELP4, Elongator Acetyltransferase Complex Subunit 4, hELP4, PAX6 neighbor gene protein, C11orf19, PAXNEB, dJ68P15A.1, PAX6NEB.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAAVATC GSVAASTGSA VATASKSNVT SFQRRGPRAS VTNDSGPRLV SIAGTRPSVR NGQLLVSTGL PALDQLLGGG LAVGTVLLIE EDKYNIYSPL LFKYFLAEGI VNGHTLLVAS AKEDPANILQ ELPAPLLDDK CKKEFDEDVY NHKTPESNIK MKIAWRYQLL PKMEIGPVSS SRFGHYYDAS KRMPQELIEA SNWHGFFLPE KISSTLKVEP CSLTPGYTKL LQFIQNIIYE EGFDGSNPQK KQRNILRIGI QNLGSPLWGD DICCAENGGN SHSLTKFLYV LRGLLRTSLS ACIITMPTHL IQNKAIIARV TTLSDVVVGL ESFIGSERET NPLYKDYHGL IHIRQIPRLN NLICDESDVK DLAFKLKRKL FTIERLHLPP DLSDTVSRSS KMDLAESAKR LGPGCGMMAG GKKHLDF.

Product Science Overview

Function and Role

The elongator complex, including ELP4, is essential for multiple tRNA modifications, such as mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) . These modifications are critical for the proper functioning of tRNA and, consequently, for protein synthesis.

ELP4 also plays a role in the formation of carboxymethyluridine in the wobble base at position 34 in tRNAs . This modification is vital for the accurate decoding of the genetic code during translation.

Genetic and Clinical Significance

The ELP4 gene has been associated with several genetic disorders. Notably, mutations in this gene have been linked to Rolandic epilepsy, a common form of childhood epilepsy . This association underscores the importance of ELP4 in neurological development and function.

Research and Applications

Research on ELP4 has provided insights into its role in transcriptional regulation and tRNA modification. The human recombinant form of ELP4 is used in various studies to understand its function and to develop potential therapeutic interventions for related genetic disorders.

In summary, ELP4 is a vital component of the elongator complex, playing a significant role in transcriptional elongation and tRNA modification. Its association with genetic disorders like Rolandic epilepsy highlights its importance in human health and disease .

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