EIF4EBP2 Human
Eukaryotic Translation Initiation Factor 4E Binding Protein 2, 4E-BP2, eIF4E-binding protein 2, 4EBP2, PHASII, phosphorylated.
Description
EIF4EBP2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Eukaryotic Translation Initiation Factor 4E Binding Protein 2, 4E-BP2, eIF4E-binding protein 2, 4EBP2, PHASII, phosphorylated.
Product Science Overview
Eukaryotic Translation Initiation Factor 4E-Binding Protein 2 (4E-BP2) is a crucial regulatory protein involved in the control of protein synthesis in eukaryotic cells. It is part of the family of 4E-Binding Proteins (4E-BPs) that interact with the eukaryotic translation initiation factor 4E (eIF4E), a key player in the initiation of cap-dependent translation. The human recombinant form of 4E-BP2 is often used in research to study its function and regulation.
4E-BP2 is a small protein that functions as a repressor of translation initiation. It achieves this by binding to eIF4E, thereby preventing the assembly of the eIF4F complex, which is essential for the recruitment of ribosomes to the mRNA cap structure . The hypophosphorylated form of 4E-BP2 has a high affinity for eIF4E and competes with eIF4G1 and eIF4G3 for binding to eIF4E . This interaction is crucial for regulating the rate of protein synthesis in response to various cellular signals.
The activity of 4E-BP2 is tightly regulated by phosphorylation. Under normal physiological conditions, 4E-BP2 is phosphorylated by kinases such as mTOR (mechanistic target of rapamycin), which reduces its affinity for eIF4E and allows the formation of the eIF4F complex . Conversely, in response to stress or nutrient deprivation, 4E-BP2 becomes hypophosphorylated, leading to its binding to eIF4E and inhibition of translation initiation .
4E-BP2 plays a significant role in various biological processes, including synaptic plasticity, learning, and memory formation . By regulating the availability of eIF4E, 4E-BP2 influences the translation of specific mRNAs that are critical for these processes. Dysregulation of 4E-BP2 has been implicated in several neuropsychiatric disorders, highlighting its importance in maintaining normal brain function .
The human recombinant form of 4E-BP2 is widely used in research to study its biochemical properties and regulatory mechanisms. It serves as a valuable tool for investigating the role of translation initiation in cellular physiology and disease. Researchers use recombinant 4E-BP2 to dissect the signaling pathways that control its activity and to identify potential therapeutic targets for conditions associated with aberrant protein synthesis.
