EIF4A3 Human

Eukaryotic Translation Initiation Factor 4A3 Human Recombinant
Cat. No.
BT4838
Source
Escherichia Coli.
Synonyms
Eukaryotic initiation factor 4A-III, eIF-4A-III, eIF4A-III, ATP-dependent RNA helicase DDX48, ATP-dependent RNA helicase eIF4A-3, DEAD box protein 48, Eukaryotic initiation factor 4A-like NUK-34, Eukaryotic translation initiation factor 4A isoform 3, Nuclear matrix protein 265, NMP 265, hNMP 265, EIF4A3, DDX48, KIAA0111, NUK34, NMP265, eIF4AIII.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

EIF4A3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 435 amino acids (1-411 a.a.) and having a molecular mass of 49.4kDa.
EIF4A3 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Eukaryotic initiation factor 4A-III (EIF4A3) belongs to the DEAD box helicase family and the eIF4A subfamily. DEAD box proteins, characterized by the conserved Asp-Glu-Ala-Asp (DEAD) motif, are believed to be RNA helicases. These proteins are involved in various cellular processes, including the modification of RNA secondary structure for translation initiation, nuclear and mitochondrial splicing, and the assembly of ribosomes and spliceosomes. EIF4A3 is a component of the exon junction complex (EJC), a splicing-dependent multiprotein complex that assembles at splice junctions on mRNAs. The distribution patterns of some DEAD box helicase family members suggest their involvement in embryogenesis, spermatogenesis, and cellular growth and division.
Description
Recombinant human EIF4A3, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 435 amino acids (amino acids 1-411) with a molecular weight of 49.4 kDa. The protein includes a 24-amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The EIF4A3 protein solution (0.5 mg/ml) is supplied in 20 mM Tris-HCl buffer (pH 8.0) containing 2 mM DTT, 30% glycerol, and 200 mM NaCl.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For long-term storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 95.0% by SDS-PAGE analysis.
Synonyms
Eukaryotic initiation factor 4A-III, eIF-4A-III, eIF4A-III, ATP-dependent RNA helicase DDX48, ATP-dependent RNA helicase eIF4A-3, DEAD box protein 48, Eukaryotic initiation factor 4A-like NUK-34, Eukaryotic translation initiation factor 4A isoform 3, Nuclear matrix protein 265, NMP 265, hNMP 265, EIF4A3, DDX48, KIAA0111, NUK34, NMP265, eIF4AIII.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMATTAT MATSGSARKR LLKEEDMTKV EFETSEEVDV TPTFDTMGLR EDLLRGIYAY GFEKPSAIQQ RAIKQIIKGR DVIAQSQSGT GKTATFSISV LQCLDIQVRE TQALILAPTR ELAVQIQKGL LALGDYMNVQ CHACIGGTNV GEDIRKLDYG QHVVAGTPGR VFDMIRRRSL RTRAIKMLVL DEADEMLNKG FKEQIYDVYR YLPPATQVVL ISATLPHEIL EMTNKFMTDP IRILVKRDEL TLEGIKQFFV AVEREEWKFD TLCDLYDTLT ITQAVIFCNT KRKVDWLTEK MREANFTVSS MHGDMPQKER ESIMKEFRSG ASRVLISTDV WARGLDVPQV SLIINYDLPN NRELYIHRIG RSGRYGRKGV AINFVKNDDI RILRDIEQYY STQIDEMPMN VADLI.

Product Science Overview

Introduction

Eukaryotic Translation Initiation Factor 4A3 (EIF4A3) is a crucial protein involved in the initiation of translation in eukaryotic cells. It is a member of the DEAD-box protein family, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). These proteins are putative RNA helicases, playing significant roles in various cellular processes that involve the alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly .

Structure and Function

EIF4A3 is an ATP-dependent RNA helicase that unwinds RNA secondary structures, facilitating the binding of ribosomes to mRNA. This process is essential for the initiation of translation. The protein is highly similar in amino acid sequence to other members of the DEAD-box protein family, such as eIF4AI and eIF4AII .

Role in Cellular Processes

EIF4A3 is a nuclear matrix protein and a core component of the exon junction complex (EJC). It participates in post-transcriptional gene regulation by promoting EJC control of precursor mRNA splicing, thus influencing nonsense-mediated mRNA decay . Additionally, EIF4A3 maintains the expression of significant selenoproteins, including phospholipid hydroperoxide glutathione peroxidase and thioredoxin reductase 1 .

Involvement in Disease

Several studies have shown that EIF4A3 is highly expressed in various human cancers, such as glioblastoma, hepatocellular carcinoma, pancreatic cancer, and ovarian cancer . It can be recruited by long non-coding RNAs to stabilize proteins and promote tumorigenesis. The overexpression of EIF4A3 has been associated with tumor growth and progression, making it a potential target for cancer therapy .

Potential as a Therapeutic Target

Given its involvement in critical cellular processes and its overexpression in tumors, EIF4A3 is being studied as a potential diagnostic biomarker, therapeutic target, and prognosis indicator for various cancers . Understanding the molecular mechanisms underlying EIF4A3’s functions and its interactions with other proteins could provide new insights into cancer treatment strategies.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

EIF4E Human
Eukaryotic Translation Initiation Factor 4E Human Recombinant
Cat. No.
BT4932
Source
Escherichia Coli.
EIF4EBP1 Human
Eukaryotic translation initiation factor 4E-binding protein 1 Human Recombinant
Cat. No.
BT5097
Source
Escherichia Coli.
EIF4EBP2 Human
Eukaryotic Translation Initiation Factor 4E-Binding Protein 2 Human Recombinant
Cat. No.
BT5175
Source
Escherichia Coli.
EIF4H Human
Eukaryotic Translation Initiation Factor 4H Human Recombinant
Cat. No.
BT5304
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.