EGFL6 Mouse
Sf9, Insect cells.
Epidermal growth factor-like protein 6, EGF-L6, Egfl6, Maeg.
Sterile filtered colorless solution.
Greater than 95.0% as determined by SDS-PAGE.
Description
EGFL6 Mouse Recombinant produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 273 amino acids (287-550a.a.) and having a molecular mass of 31.1kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
EGFL6 is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Epidermal growth factor-like protein 6, EGF-L6, Egfl6, Maeg.
Sf9, Insect cells.
ADLTMKKKVK LKMVTPRPAS TRVPKVNLPY SSEEGVSRGR NYDGEQKKKE EGKRERLEEE
KGEKTLRNEV EQERTLRGDV FSPKVNEAED LDLVYVQRKE LNSKLKHKDL NISVDCSFDL
GVCDWKQDRE DDFDWHPADR DNDVGYYMAV PALAGHKKNI GRLKLLLPNL TPQSNFCLLF
DYRLAGDKVG KLRVFVKNSN NALAWEETKN EDGRWRTGKI QLYQGIDTTK SVIFEAERGK GKTGEIAVDG VLLVSGLCPD DFLSVEGHHH HHH.
Product Science Overview
EGFL6 is a secreted protein that contains several key structural features:
- Signal Peptide: This sequence suggests that EGFL6 is secreted outside the cell.
- EGF-like Repeats: The protein has four complete EGF-like repeats and one partial EGF-like repeat. Three of these repeats have a calcium-binding consensus sequence, which is crucial for their function.
- RGD Motif: This arginine-glycine-aspartic acid sequence is known to bind integrins, which are receptors that mediate cell adhesion.
- MAM Domain: This domain is believed to have an adhesive function, contributing to the protein’s role in cell adhesion and signaling .
EGFL6 plays a significant role in various biological processes:
- Cell Cycle Regulation: It is involved in the regulation of the cell cycle, ensuring proper cell division and proliferation.
- Developmental Processes: EGFL6 is expressed early during development and has been detected in various fetal tissues such as the lung, heart, liver, spleen, cochlea, and placenta .
- Tumor Expression: Its expression has also been observed in certain tumors, including lung and meningioma tumors, suggesting a potential role in tumorigenesis .
Recombinant mouse EGFL6 is produced through genetic engineering techniques, providing a valuable tool for investigating its biological functions and potential therapeutic applications. The recombinant protein is typically produced in human embryonic kidney (HEK) cells and purified using proprietary chromatographic techniques .
- Physical Appearance: The recombinant protein is usually provided as a sterile filtered white lyophilized (freeze-dried) powder.
- Formulation: It is lyophilized from a solution containing 20mM MES and 500mM NaCl, pH 6.0, with 5% trehalose.
- Stability: The lyophilized protein is stable at room temperature for three weeks but should be stored desiccated below -18°C for long-term storage. Upon reconstitution, it should be stored at 4°C for short-term use .
Recombinant mouse EGFL6 is used in various research applications:
- Cell Adhesion Studies: Its ability to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells is a key area of study.
- Developmental Biology: Researchers use it to study its role in early development and tissue formation.
- Cancer Research: Given its expression in tumors, EGFL6 is investigated for its potential role in cancer progression and as a therapeutic target .
