EGFL6 Human
EGF Like Domain Multiple 6 Human Recombinant
Cat. No.
BT4116
Source
HEK (Human embryonic kidney cells).
Synonyms
EGF Like Domain Multiple 6, MAM and EGF Domains-Containing Gene Protein, MAM and EGF Domain Containing, EGF-Like Protein 6, MAEG, EGF Repeat-Containing Protein 6, W80, EGFL6.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
EGF Like Domain Multiple 6 Human Recombinant produced in HEK cells is a polypeptide chain starting at amino acid Asn at position 22 to amino acid Arg at position 363, fused to an FC, 6 x His-tag at C-terminus, containing a total of 348 amino acids and having a predicted molecular mass of 40-55kDa. The EGFL6 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Epidermal Growth Factor-like Domain Multiple 6 (EGFL6), a member of the EGF repeat superfamily, plays a crucial role in regulating cell cycle progression, proliferation, and developmental processes. This secreted protein is characterized by a signal peptide, an EGF repeat region comprising four complete and one partial EGF-like repeats (three with a calcium-binding consensus sequence), an arg-gly-asp integrin association motif, and a MAM domain, which is believed to have adhesive properties. Human EGFL6 shares significant amino acid sequence identity with its mouse and rat counterparts (75% and 78%, respectively). During early development, EGFL6 is expressed in various fetal tissues, including the lung, heart, liver, spleen, cochlea, and placenta, as well as in meningioma tumors.
Description
Recombinant Human EGFL6, produced in HEK cells, is a polypeptide chain spanning from amino acid Asn at position 22 to amino acid Arg at position 363. It is fused to an FC and a 6 x His-tag at the C-terminus, resulting in a total of 348 amino acids. With a predicted molecular mass of 40-55kDa, the EGFL6 protein undergoes purification using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The EGFL6 protein was lyophilized from a 0.2µm filtered solution containing 20mM MES, 500mM NaCl, pH 6.0, and 5% Trehalose.
Solubility
To reconstitute the lyophilized EGFL6, it is recommended to dissolve it in sterile PBS at a concentration of 500µg/ml. This solution can then be further diluted in other aqueous solutions as needed.
Stability
Lyophilized EGFL6 remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated below -18°C. After reconstitution, EGFL6 should be stored at 4°C for 2-7 days. For future use, store below -18°C. To ensure optimal stability during long-term storage, consider adding a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Purity
The purity of EGFL6 is determined to be greater than 95.0% using SDS-PAGE analysis.
Biological Activity
The biological activity of EGFL6 is assessed by its ability to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells when immobilized. The expected ED50 for this effect is in the range of 1-5 µg/ml.
Synonyms
EGF Like Domain Multiple 6, MAM and EGF Domains-Containing Gene Protein, MAM and EGF Domain Containing, EGF-Like Protein 6, MAEG, EGF Repeat-Containing Protein 6, W80, EGFL6.
Source
HEK (Human embryonic kidney cells).
Product Science Overview
Gene and Protein Structure
The EGFL6 gene encodes a protein that contains several distinct domains:
- Signal Peptide: Suggests that the protein is secreted.
- EGF Repeat Region: Consists of four complete EGF-like repeats and one partial EGF-like repeat. Three of these repeats have a calcium-binding consensus sequence.
- Arg-Gly-Asp (RGD) Integrin Association Motif: This motif is known to bind integrins, which are receptors that facilitate cell-extracellular matrix adhesion.
- MAM Domain: Believed to have an adhesive function .
Expression and Function
Clinical Relevance
Recombinant Human EGF-L6
Recombinant human EGF-L6 protein is produced using human embryonic kidney cell (HEK293)-derived systems. The protein is purified to a high degree, with a purity greater than 95% as determined by SDS-PAGE . It is used in various research applications, including studies on cell adhesion and matrix assembly .
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