EGF Antibody
Endothelial Growth Factor, Mouse Anti-Human
Cat. No.
BT4431
Source
Synonyms
Urogastrone, URG, EGF.
Appearance
Purity
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Product Specs
Introduction
Epidermal growth factor (EGF) is a crucial signaling molecule that plays a significant role in cell differentiation and proliferation. It exerts a strong mitogenic effect on various cultured cells, including those of ectodermal and mesodermal origin. The EGF precursor is thought to be a membrane-bound molecule that undergoes proteolytic cleavage to release the active 53-amino acid peptide hormone, which then stimulates cell division. EGF promotes the growth of different epidermal and epithelial tissues both in living organisms and laboratory settings, as well as certain fibroblasts in cell culture.
Formulation
Supplied as a 1mg/ml solution in phosphate-buffered saline (PBS) upon reconstitution.
Shipping Conditions
To ensure stability during transportation, the antibody is shipped in lyophilized form at ambient temperature.
Storage Procedures
For long-term storage, keep the lyophilized antibody at 4 degrees Celsius in a dry environment. Once reconstituted, if not used within a month, aliquot the antibody and store at -20 degrees Celsius.
Solubility
To reconstitute the antibody, add sterile water to the vial. Gently mix the solution, ensuring the contents are dissolved by washing the sides of the vial. Allow 30-60 seconds for complete reconstitution before use.
Applications
This antibody is suitable for various immunological techniques, including direct enzyme-linked immunosorbent assay (ELISA), Western blotting, and immunoprecipitation.
Titer
When used in a direct ELISA, a 1:10,000 dilution of the antibody exhibits an optical density (O.D.) of 0.3 when detected with an alkaline phosphatase-conjugated rabbit anti-mouse immunoglobulin G (IgG) secondary antibody from Jackson Laboratories.
Synonyms
Urogastrone, URG, EGF.
Purification Method
Ion exchange.
Type
Mouse Anti Human Monoclonal.
Clone
NYRhEGF.
Immunogen
r.HumanEGF.
Ig Subclass
Mouse IgG2b.
Product Science Overview
VEGF and Its Importance
VEGF is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis, promoting tumor progression and metastasis . It has a variety of effects on vascular endothelium, including the ability to promote endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability . The VEGF family includes several members such as VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and Placenta Growth Factor (PIGF) .
Mouse Anti-Human VEGF Antibodies
Mouse anti-human VEGF antibodies are monoclonal antibodies developed in mice that specifically target human VEGF. These antibodies are used in various scientific applications, including immunohistochemistry, Western blot, immunocytochemistry, ELISA, and flow cytometry . They are crucial tools in research for detecting and quantifying VEGF in human samples.
Applications in Research
- Cancer Research: VEGF is a key player in tumor angiogenesis. By targeting VEGF, researchers can study its role in tumor growth and metastasis. Anti-VEGF therapies are being developed to inhibit tumor growth by blocking the VEGF signaling pathway .
- Cardiovascular Research: VEGF is also involved in the formation of new blood vessels in response to ischemia. Researchers use mouse anti-human VEGF antibodies to study cardiovascular diseases and develop potential treatments .
- Wound Healing: VEGF plays a role in wound healing by promoting the formation of new blood vessels. Researchers study VEGF to understand its role in tissue repair and develop therapies to enhance wound healing .
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