EBV EBNA1 Mosaic

The Epstein-Barr Virus (EBV), also known as Human Herpesvirus 4 (HHV-4), is a member of the herpesvirus family. It is one of the most common viruses in humans and is best known for causing infectious mononucleosis, also known as “mono” or the “kissing disease.” EBV is also associated with various types of cancers, including Burkitt’s lymphoma, Hodgkin’s lymphoma, and nasopharyngeal carcinoma.

The Epstein-Barr Virus Nuclear Antigen 1 (EBNA1) is a crucial protein expressed in all EBV-associated malignancies. EBNA1 plays a vital role in the replication and maintenance of the viral genome within infected cells. It is also involved in the regulation of viral gene expression. The protein contains a glycine-alanine repeat region that helps it evade the host’s immune system by preventing proper processing and presentation through the major histocompatibility complex class I (MHC I) pathway .

The EBNA1 Mosaic Recombinant is a specially designed protein that includes specific regions of the EBNA1 protein. This recombinant protein is typically produced in E. coli and contains the HHV-4 EBNA1 regions, specifically amino acids 1-90 and 408-498 . The molecular weight of this recombinant protein is approximately 46 kDa, including a GST tag .

The EBNA1 Mosaic Recombinant protein is highly immunoreactive with sera from EBV-infected individuals, making it an excellent antigen for use in various immunological assays, such as ELISA and Western blots . It is particularly useful for detecting EBV infections with minimal specificity problems. Additionally, the recombinant protein is used in research to study the mechanisms of EBV-related diseases and to develop potential therapeutic strategies.

The EBNA1 Mosaic Recombinant protein is typically purified using proprietary chromatographic techniques and has a purity of over 95% as determined by SDS-PAGE . For optimal stability, the protein should be stored at -20°C and should avoid freeze-thaw cycles to maintain its integrity .