EBV EBNA1
Escherichia Coli.
Epstein-Barr virus nuclear antigen 1, BKRF1, EBNA1.
Filtered White lyophilized (freeze-dried) powder.
Greater than 95% as determined by SDS-PAGE.
Description
EBV EBNA1 Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 242 amino acids (His407-Glu641) and having a molecular mass of 25.9kDa.
EBV EBNA1 is fused to a 7 a.a his tag at N-Terminus and is purified by proprietary chromatographic techniques.
Product Specs
Epstein-Barr virus nuclear antigen 1, BKRF1, EBNA1.
Escherichia Coli.
MKHHHHHHPV GEADYFEYHQ EGGPDGEPDV PPGAIEQGPA DDPGEGPSTG PRGQGDGGRR KKGGWFGKHR GQGGSNPKFE NIAEGLRALL ARSHVERTTD EGTWVAGVFV YGGSKTSLYN LRRGTALAIP QCRLTPLSRL PFGMAPGPGP QPGPLRESIV CYFMVFLQTH IFAEVLKDAI KDLVMTKPAP TCNIRVTVCS FDDGVDLPPW FPPMVEGAAA EGDDGDDGDE GGDGDEGEEG QE.
Product Science Overview
The Epstein-Barr Virus (EBV), also known as Human Herpesvirus 4 (HHV-4), is a member of the herpesvirus family. It is one of the most common viruses in humans and is known for its ability to establish lifelong latent infections in B lymphocytes . EBV is associated with various malignancies, including nasopharyngeal carcinoma, Hodgkin’s lymphoma, and Burkitt’s lymphoma .
The Epstein-Barr Virus Nuclear Antigen 1 (EBNA1) is a crucial viral protein expressed in all EBV-associated tumors and latency programs . EBNA1 plays a vital role in the replication and maintenance of the viral episome within infected cells . It is the only EBV protein consistently expressed in all forms of EBV-related malignancies .
EBNA1 contains a glycine-alanine repeat region that inhibits its processing and presentation by the major histocompatibility complex class I (MHC I) pathway . This allows EBNA1 to evade immune detection, contributing to the persistence of the virus in the host. Additionally, EBNA1 has been shown to modulate the alternative splicing of cellular genes, which may play a role in its oncogenic activity .
Recombinant EBNA1 proteins are produced using various expression systems, such as E. coli, to study the protein’s structure and function. These recombinant proteins are often used as antigens in ELISA and Western blot assays to detect EBV infections . The recombinant EBNA1 protein typically includes specific fragments of the native protein, such as amino acids 1-90 and 408-498 .
Recombinant EBNA1 proteins are valuable tools in research and diagnostic applications. They are used to study the mechanisms of EBV latency and oncogenesis, as well as to develop diagnostic assays for detecting EBV infections . Additionally, recombinant EBNA1 proteins can be used to investigate the immune response to EBV and to develop potential therapeutic strategies targeting EBNA1 .
