DYNLRB2 Human
Dynein Light Chain Roadblock-Type 2 Human Recombinant
Cat. No.
BT540
Source
Escherichia Coli.
Synonyms
Dynein light chain roadblock-type 2, Dynein light chain 2B cytoplasmic, Roadblock domain-containing protein 2, DYNLRB2, DNCL2B, DNLC2B, ROBLD2, MGC62033.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
DYNLRB2 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 116 amino acids (1-96 a.a.) and having a molecular mass of 13kDa. The DYNLRB2 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Dynein light chain roadblock-type 2 (DYNLRB2) is a non-catalytic component of the cytoplasmic dynein complex, which is responsible for the intracellular retrograde transport of vesicles and organelles along microtubules. DYNLRB2 is believed to play a role in connecting dynein to its cargo and to regulatory adapter proteins.
Description
Recombinant human DYNLRB2, expressed in E. coli with an N-terminal 20 amino acid His tag, is a non-glycosylated polypeptide chain consisting of 116 amino acids (residues 1-96 of the DYNLRB2 sequence) with a molecular weight of 13 kDa. Purification is achieved using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution, sterile filtered.
Formulation
DYNLRB2 is supplied as a 0.5 mg/ml solution in 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, and 1mM DTT.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the product frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for extended storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
Dynein light chain roadblock-type 2, Dynein light chain 2B cytoplasmic, Roadblock domain-containing protein 2, DYNLRB2, DNCL2B, DNLC2B, ROBLD2, MGC62033.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAEVEETLKR IQSHKGVIGT MVVNAEGIPI RTTLDNSTTV QYAGLLHHLT MKAKSTVRDI DPQNDLTFLR IRSKKHEIMV APDKEYLLIV IQNPCE.
Product Science Overview
Gene and Protein Structure
Function
Dynein Light Chain Roadblock-Type 2 functions as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex . This complex acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules . The protein is thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function .
Expression and Localization
Industrial Production
Recombinant Human DYNLRB2 protein is produced using Escherichia coli as the expression system . The protein is purified using anion-exchange chromatography and gel-filtration chromatography to achieve a purity of over 90% . This recombinant protein is suitable for various applications, including SDS-PAGE and mass spectrometry .
Clinical Relevance
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