DLK1 Human, HEK

Delta-Like 1 Human Recombinant, HEK
Cat. No.
BT4635
Source

HEK293 Cells.

Synonyms

DLK1, Delta-Like 1 Homolog (Drosophila), PREF1, DLK-1, FA1, DLK, PG2, Delta-Like Homolog (Drosophila), Protein Delta Homolog 1, Preadipocyte Factor 1, Fetal Antigen 1, Secredeltin, DELTA1, Pref-1, ZOG.

Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

DLK1 Human Recombinant produced in HEK293 Cells is a single, glycosylated, polypeptide chain (a.a 24-303) containing 290 amino acids including a 10 a.a C-terminal His tag. The total molecular mass is 31.2kDa (calculated). 

Product Specs

Introduction
Delta-Like 1, also referred to as DLK1, belongs to the Notch/Delta/Serrate protein family. These proteins are characterized by epidermal growth factor (EGF)-like repeats and function as ligands in the Notch signaling pathway. DLK1 possesses six highly conserved EGF motifs and contains up to ten potential glycosylation sites, with six exhibiting differential glycosylation patterns.
Description
Recombinant human DLK1, expressed in HEK293 cells, is a single, glycosylated polypeptide chain. It encompasses amino acids 24 to 303, totaling 290 amino acids, and includes a 10-amino acid C-terminal His tag. The calculated molecular mass is 31.2 kDa.
Physical Appearance
White, lyophilized powder (freeze-dried) that has been filtered.
Formulation
DLK1 is filtered through a 0.4 μm filter and subsequently lyophilized from a solution of PBS (pH 7.4) containing 5% w/v trehalose at a concentration of 0.5 mg/ml.
Solubility
To create a working stock solution, it is advised to reconstitute the lyophilized pellet with deionized water to an approximate concentration of 0.5 mg/ml. Ensure complete dissolution of the pellet. Prior to use in cell culture, it's crucial to sterilize the product by filtration using an appropriate sterile filter as DLK1 is not supplied sterile.
Stability
Store the lyophilized protein at -20°C. Once reconstituted, aliquot the protein to minimize freeze-thaw cycles. The reconstituted protein demonstrates stability at 4°C for a limited period and remains stable for up to two weeks when stored at this temperature.
Purity
Purity exceeds 95.0% as assessed by SDS-PAGE analysis.
Synonyms

DLK1, Delta-Like 1 Homolog (Drosophila), PREF1, DLK-1, FA1, DLK, PG2, Delta-Like Homolog (Drosophila), Protein Delta Homolog 1, Preadipocyte Factor 1, Fetal Antigen 1, Secredeltin, DELTA1, Pref-1, ZOG.

Source

HEK293 Cells.

Amino Acid Sequence

AECFPACNPQ NGFCEDDNVC RCQPGWQGPL CDQCVTSPGC LHGLCGEPGQ CICTDGWDGE LCDRDVRACS SAPCANNRTC VSLDDGLYEC SCAPGYSGKD CQKKDGPCVI NGSPCQHGGT CVDDEGRASH ASCLCPPGFS GNFCEIVANS CTPNPCENDG VCTDIGGDFR CRCPAGFIDK TCSRPVTNCA SSPCQNGGTC LQHTQVSYEC LCKPEFTGLT CVKKRALSPQ QVTRLPSGYG LAYRLTPGVH ELPVQQPEHR ILKVSMKELN KKTPLLTEGQ HHHHHHHHHH.

Product Science Overview

Structure and Characteristics

DLK1 is a transmembrane protein that can also exist in a soluble form. It contains six highly conserved EGF-like motifs and multiple glycosylation sites, which are essential for its function and stability . The recombinant form of DLK1, expressed in HEK 293 cells, is a glycoprotein with a molecular weight of approximately 57.0-60.0 kDa . This recombinant protein is often used in research due to its high purity and suitability for cell culture applications .

Biological Function

DLK1 plays a significant role in various biological processes:

  • Notch Signaling Pathway: DLK1 functions as a ligand for Notch receptors, specifically activating Notch-1 and Notch-2. This pathway is vital for the development, maintenance, and remodeling of the vascular system .
  • Hematopoiesis: DLK1 suppresses the differentiation of hematopoietic progenitor cells into the B-cell lineage while promoting their differentiation into T-cell and natural killer (NK) cell precursors .
  • Organ Development: DLK1 is expressed in the heart and pancreas and plays a role in the development of these organs .
Applications in Research

Recombinant DLK1 is widely used in laboratory research to study its role in the Notch signaling pathway and its effects on cell differentiation and proliferation. It is also used to investigate its potential therapeutic applications in diseases where Notch signaling is dysregulated .

Storage and Stability

DLK1 protein solution is typically stored at 4°C for short-term use (2-4 weeks) and at -20°C for long-term storage. It is recommended to add a carrier protein, such as human serum albumin (HSA) or bovine serum albumin (BSA), to prevent degradation during storage .

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