DLK1 Human
Delta-Like 1 Human Recombinant
Cat. No.
BT4558
Source
Sf9, Baculovirus cells.
Synonyms
DLK1, Delta-Like 1 Homolog (Drosophila), PREF1, DLK-1, FA1, DLK, PG2, Delta-Like Homolog (Drosophila), Protein Delta Homolog 1, Preadipocyte Factor 1, Fetal Antigen 1, Secredeltin, DELTA1, Pref-1, ZOG.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
DLK1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 288 amino acids (24-303 a.a) and having a molecular mass of 30.7kDa.
DLK1 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Delta-Like 1, also called DLK1, is a protein that belongs to the Notch/Delta/Serrate family. This family is known for its role in the Notch signaling pathway, where the proteins act as ligands (like keys) that activate the pathway. DLK1 has six special regions called epidermal-growth-factor motifs, which are important for its function. It also has specific sites where sugar molecules can attach (glycosylation), and these sugar modifications can vary.
Description
This product contains human DLK1 protein made in insect cells (Sf9 Baculovirus). It's a single chain of 288 amino acids, with a molecular weight of 30.7kDa. The protein has a small tag (His-tag) attached to aid in purification, and it's purified to a high degree. The protein is glycosylated, meaning it has sugars attached, which is important for its natural properties.
Physical Appearance
The product appears as a clear liquid that has been sterilized by filtration.
Formulation
The DLK1 protein is provided in a solution at a concentration of 0.25mg/ml. The solution contains Phosphate Buffered Saline (PBS) with a pH of 7.4 and 10% glycerol for stability.
Stability
For short-term storage (up to 4 weeks), keep the product refrigerated at 4°C. For longer storage, freeze the product at -20°C. It's best to add a carrier protein (0.1% HSA or BSA) before freezing to protect the DLK1 protein. Avoid repeatedly freezing and thawing the product as this can damage the protein.
Purity
The purity of the DLK1 protein is greater than 95%, as determined by SDS-PAGE, a common method for analyzing protein purity.
Synonyms
DLK1, Delta-Like 1 Homolog (Drosophila), PREF1, DLK-1, FA1, DLK, PG2, Delta-Like Homolog (Drosophila), Protein Delta Homolog 1, Preadipocyte Factor 1, Fetal Antigen 1, Secredeltin, DELTA1, Pref-1, ZOG.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
AECFPACNPQ NGFCEDDNVC RCQPGWQGPL CDQCVTSPGC LHGLCGEPGQ CICTDGWDGE LCDRDVRACS SAPCANNGTC VSLDDGLYEC SCAPGYSGKD CQKKDGPCVI NGSPCQHGGT CVDDEGRASH ASCLCPPGFS GNFCEIVANS CTPNPCENDG VCTDIGGDFR CRCPAGFIDK TCSRPVTNCA SSPCQNGGTC LQHTQVSYEC LCKPEFTGLT CVKKRALSPQ QVTRLPSGYG LAYRLTPGVH ELPVQQPEHR ILKVSMKELN KKTPLLTEGQ LEHHHHHH.
Product Science Overview
Structure and Composition
Mature human DLL1 consists of:
- A 528 amino acid extracellular domain (ECD) with one DSL domain and eight EGF-like repeats
- A 23 amino acid transmembrane segment
- A 155 amino acid cytoplasmic domain
The protein is widely expressed and localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 .
Functions
DLL1 is essential for:
- Embryonic Development: It plays a significant role in embryonic somite formation and cochlear hair cell differentiation .
- Immune System: It is involved in the differentiation of B and T lymphocytes .
- Vascular System: The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis .
Clinical Significance
Recombinant DLL1
Storage and Handling
- Lyophilized Form: Stable for up to 12 months when stored at -20 to -80°C.
- Reconstituted Form: Can be stored at 4-8°C for 2-7 days or at < -20°C for up to 3 months .
DLL1’s role in cell fate decisions and its involvement in critical developmental and pathological processes make it a significant protein for research in developmental biology and oncology.
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