Dengue NS1 ST3, Insect
Description
Dengue NS1 ST3 is purified by proprietary chromatographic technique.
Product Specs
Product Science Overview
Dengue virus (DENV) is a mosquito-borne flavivirus that causes dengue fever, a severe flu-like illness. The virus has four serotypes (DENV1-4), each capable of causing the disease. Among the various proteins encoded by the dengue virus, the non-structural protein 1 (NS1) plays a crucial role in viral replication, assembly, pathogenesis, and immune evasion .
The NS1 protein is a glycoprotein that is secreted by infected cells and can be found in the blood of infected individuals. It is involved in several key processes:
- Viral Replication: NS1 is essential for the replication of the viral RNA genome.
- Immune Evasion: NS1 helps the virus evade the host’s immune system by interfering with the complement pathway.
- Pathogenesis: High levels of NS1 in the blood are associated with severe forms of dengue, such as dengue hemorrhagic fever and dengue shock syndrome .
Recombinant NS1 proteins are produced using various expression systems, including bacterial, yeast, insect, and mammalian cells. The use of insect cells for the production of recombinant NS1 protein has several advantages:
- Post-Translational Modifications: Insect cells can perform post-translational modifications similar to those in mammalian cells, which are essential for the proper folding and function of the NS1 protein.
- High Yield: Insect cells can produce high yields of recombinant proteins.
- Safety: The use of insect cells reduces the risk of contamination with human pathogens .
Dengue virus subtype 3 (DENV3) is one of the four serotypes of the dengue virus. The NS1 protein of DENV3 has been extensively studied for its role in the immune response and its potential as a diagnostic and therapeutic target. Recombinant NS1 proteins from DENV3 have been used in various research and diagnostic applications .
The production of recombinant NS1 protein in insect cells involves several steps:
- Gene Cloning: The gene encoding the NS1 protein is cloned into an expression vector.
- Transfection: The expression vector is introduced into insect cells, such as Spodoptera frugiperda (Sf9) cells.
- Protein Expression: The insect cells are cultured, and the NS1 protein is expressed.
- Protein Purification: The recombinant NS1 protein is purified from the cell culture supernatant using techniques such as affinity chromatography .
Recombinant NS1 proteins have several important applications:
- Diagnostics: NS1 proteins are used in diagnostic assays to detect dengue virus infections. These assays can identify the presence of NS1 in the blood of infected individuals, providing an early diagnosis of dengue.
- Vaccine Development: NS1 proteins are being investigated as potential components of dengue vaccines. The immunogenic properties of NS1 make it a promising candidate for inducing protective immunity against dengue virus .
- Research: Recombinant NS1 proteins are used in research to study the structure and function of the NS1 protein, as well as its interactions with the host immune system .
