Dengue Envelope-4 45kDa

Dengue fever is a mosquito-borne viral infection caused by the dengue virus, which belongs to the genus Flavivirus and the family Flaviviridae. There are four closely related but antigenically distinct serotypes of the dengue virus: DENV-1, DENV-2, DENV-3, and DENV-4. Infection with one serotype provides lifelong immunity to that specific serotype but only partial and temporary protection against the others. Subsequent infections with different serotypes increase the risk of developing severe dengue, also known as dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS).

DENV-4 is one of the four serotypes of the dengue virus. It is less commonly associated with severe disease compared to DENV-2 and DENV-3, but it can still cause significant morbidity and mortality. The envelope (E) protein of the dengue virus is a key structural protein that plays a crucial role in the virus’s ability to infect host cells. It is involved in the binding of the virus to host cell receptors and the subsequent fusion of the viral and host cell membranes.

The recombinant Dengue Virus Subtype 4 Envelope 45kDa protein is a laboratory-produced version of the E protein. This recombinant protein is typically produced in Escherichia coli (E. coli) and is fused to a 6xHis tag at the C-terminus to facilitate purification. The protein is approximately 45 kilodaltons (kDa) in size and includes amino acids 43-413 of the E protein .

The recombinant DENV-4 E protein is produced using recombinant DNA technology. The gene encoding the E protein is cloned into an expression vector, which is then introduced into E. coli cells. The bacteria are cultured, and the recombinant protein is expressed. The protein is then purified using affinity chromatography, taking advantage of the 6xHis tag. The purity of the protein is typically greater than 95%, as determined by polyacrylamide gel electrophoresis (PAGE) with Coomassie staining .

The recombinant DENV-4 E protein is used in various research and diagnostic applications. It is commonly used in serological assays to detect antibodies against the dengue virus in patient samples. These assays are important for the diagnosis of dengue fever and for epidemiological studies. The protein is also used in vaccine research, as it can elicit an immune response in animal models. Additionally, it is used in studies to understand the structure and function of the E protein and its role in the virus’s life cycle .

The recombinant DENV-4 E protein is stable at 4°C for up to one week but should be stored at temperatures below -18°C for long-term storage. It is important to avoid repeated freeze-thaw cycles to maintain the protein’s integrity .