DDX56 Human
DEAD Box Protein 56 Human Recombinant
Cat. No.
BT29115
Source
Escherichia Coli.
Synonyms
DEAD (Asp-Glu-Ala-Asp) Box Helicase 56, 61-Kd Nucleolar Helicase, DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 56, DDX26, Nucleolar Helicase Of 61 KDa, DEAD-Box RNA Helicase, DDX21, Probable ATP-Dependent RNA Helicase DDX56, NOH61, Putative Nucleolar RNA Helicase, ATP-Dependent 61 KDa Nucleolar RNA Helicase, EC 3.6.4.13, DEAD Box Protein 21, EC 3.6.1, DEAD Box Protein 56.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
DDX56 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 570 amino acids (1-547 a.a) and having a molecular mass of 64kDa.
DDX56 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
DDX56 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
DEAD Box Protein 56 (DDX56), a member of the DEAD box protein family, is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) and is believed to function as an RNA helicase. This family of proteins is involved in various cellular processes that require modification of RNA secondary structure, including translation initiation, nuclear and mitochondrial splicing, and the assembly of ribosomes and spliceosomes. The distribution patterns of some family members suggest roles in embryogenesis, spermatogenesis, and cellular growth and division. DDX56 demonstrates ATPase activity in the presence of polynucleotides and interacts with nucleoplasmic 65S preribosomal particles.
Description
Recombinant human DDX56, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 570 amino acids (residues 1-547). A 23 amino acid His-tag is fused to the N-terminus, resulting in a protein with a molecular weight of 64 kDa. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile filtered solution.
Formulation
The DDX56 protein solution (1 mg/ml) is supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 10% glycerol, and 0.4 M urea.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
Purity is determined to be greater than 85.0% via SDS-PAGE analysis.
Synonyms
DEAD (Asp-Glu-Ala-Asp) Box Helicase 56, 61-Kd Nucleolar Helicase, DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 56, DDX26, Nucleolar Helicase Of 61 KDa, DEAD-Box RNA Helicase, DDX21, Probable ATP-Dependent RNA Helicase DDX56, NOH61, Putative Nucleolar RNA Helicase, ATP-Dependent 61 KDa Nucleolar RNA Helicase, EC 3.6.4.13, DEAD Box Protein 21, EC 3.6.1, DEAD Box Protein 56.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMEDSEAL GFEHMGLDPR LLQAVTDLGW SRPTLIQEKA IPLALEGKDL LARARTGSGK TAAYAIPMLQ LLLHRKATGP VVEQAVRGLV LVPTKELARQ AQSMIQQLAT YCARDVRVAN VSAAEDSVSQ RAVLMEKPDV VVGTPSRILS HLQQDSLKLR DSLELLVVDE ADLLFSFGFE EELKSLLCHL PRIYQAFLMS ATFNEDVQAL KELILHNPVT LKLQESQLPG PDQLQQFQVV CETEEDKFLL LYALLKLSLI RGKSLLFVNT LERSYRLRLF LEQFSIPTCV LNGELPLRSR CHIISQFNQG FYDCVIATDA EVLGAPVKGK RRGRGPKGDK ASDPEAGVAR GIDFHHVSAV LNFDLPPTPE AYIHRAGRTA RANNPGIVLT FVLPTEQFHL GKIEELLSGE NRGPILLPYQ FRMEEIEGFR YRCRDAMRSV TKQAIREARL KEIKEELLHS EKLKTYFEDN PRDLQLLRHD LPLHPAVVKP HLGHVPDYLV PPALRGLVRP HKKRKKLSSS CRKAKRAKSQ NPLRSFKHKG KKFRPTAKPS.
Product Science Overview
Structure and Function
DEAD box proteins, including DDX56, are known for their ATPase activity in the presence of polynucleotides. DDX56 specifically associates with nucleoplasmic 65S preribosomal particles, indicating its involvement in ribosome synthesis, most likely during the assembly of the large 60S ribosomal subunit . The protein encoded by the DDX56 gene shows ATPase activity and is implicated in maintaining nucleolar integrity and ribosome biogenesis .
Biological Processes
DDX56 plays a role in various biological processes, including:
- Innate Immunity: It is involved in the immune response, particularly in the recognition and response to viral infections .
- Ribosome Biogenesis: DDX56 is essential for the assembly and stability of ribosomes, which are crucial for protein synthesis .
- Nucleolus Organization: It helps maintain the structure and function of the nucleolus, a subnuclear body involved in ribosome production .
Clinical Significance
Recombinant DDX56
Recombinant human DDX56 protein is often used in research to study its function and role in various cellular processes. The recombinant protein is typically expressed in E. coli and purified for use in various assays . It is available with tags such as His-tag to facilitate purification and detection .
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