DDX39A Human
Description
DDX39A is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
DEAD Box Protein 39A (DDX39A), also known as BAT1, is a member of the DEAD box protein family. These proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) and are putative RNA helicases . They play crucial roles in various cellular processes involving the alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly .
DDX39A is a single, non-glycosylated polypeptide chain containing 274 amino acids and has a molecular mass of approximately 31 kDa . The protein is involved in multiple cellular processes, including:
- Translation Initiation: DDX39A helps in the initiation of protein synthesis by unwinding RNA secondary structures that can impede the translation machinery.
- Splicing: It plays a role in both nuclear and mitochondrial splicing, ensuring the proper removal of introns from pre-mRNA and the correct assembly of exons.
- Ribosome and Spliceosome Assembly: DDX39A is essential for the assembly of ribosomes and spliceosomes, which are critical for protein synthesis and mRNA processing, respectively .
The DEAD box protein family, including DDX39A, is implicated in several vital biological processes:
- Embryogenesis: These proteins are believed to be involved in the early stages of embryonic development.
- Spermatogenesis: They play a role in the development of sperm cells.
- Cellular Growth and Division: DDX39A is crucial for cell proliferation and division, making it an important factor in tissue growth and repair .
DDX39A has been identified as a potential biomarker for unfavorable neuroblastoma, a type of cancer that arises from nerve tissue . Its expression levels can provide insights into the prognosis of patients with this condition. Additionally, a pseudogene of DDX39A is present on chromosome 13, and alternative splicing results in multiple transcript variants, although their full-length nature is not yet fully understood .
The recombinant form of DDX39A is produced in E. coli and is used for various research applications. It is a valuable tool for studying the protein’s function and its role in different cellular processes. The recombinant protein is typically fused to a tag, such as GST, to facilitate its purification and detection in experimental settings .
