DCTPP1 Human

dCTP Pyrophosphatase 1 Human Recombinant
Cat. No.
BT27316
Source
E.coli.
Synonyms
dCTP pyrophosphatase 1, RS21C6, XTP3TPA, Deoxycytidine-triphosphatase 1, dCTPase 1, XTP3-transactivated gene A protein, CDA03, MGC5627, XTP3-transactivated protein A, EC 3.6.1.12.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

DCTPP1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 194 amino acids (1-170) and having a molecular mass of 21.2kDa.
DCTPP1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
DCTPP1, also known as dCTP pyrophosphatase 1, contains an epilepsy-associated repeat (EAR) domain predicted to participate in protein-protein interactions, likely involved in ligand recognition. This protein hydrolyzes abnormal nucleotide triphosphates (NTPs) in cancer cells, which could be incorporated into nascent DNA or RNA if unregulated.
Description
Recombinant human DCTPP1, produced in E.coli, is a single, non-glycosylated polypeptide chain containing 194 amino acids (1-170) with a molecular mass of 21.2kDa. It is fused to a 24 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Colorless, sterile-filtered solution.
Formulation
The DCTPP1 solution (0.5mg/ml) is supplied in 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For long-term storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
Purity greater than 90% as determined by SDS-PAGE.
Synonyms
dCTP pyrophosphatase 1, RS21C6, XTP3TPA, Deoxycytidine-triphosphatase 1, dCTPase 1, XTP3-transactivated gene A protein, CDA03, MGC5627, XTP3-transactivated protein A, EC 3.6.1.12.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSVAGG EIRGDTGGED TAAPGRFSFS PEPTLEDIRR LHAEFAAERD WEQFHQPRNL LLALVGEVGE LAELFQWKTD GEPGPQGWSP RERAALQEEL SDVLIYLVAL AARCRVDLPL AVLSKMDINR RRYPAHLARS SSRKYTELPH GAISEDQAVG PADIPCDSTG QTST.

Product Science Overview

Function and Mechanism

DCTPP1 exhibits a strong preference for dCTP and its analogs, including 5-iodo-dCTP and 5-methyl-dCTP . The enzyme also displays weak activity against deoxythymidine triphosphate (dTTP) and deoxyadenosine triphosphate (dATP), but it does not act on deoxyguanosine triphosphate (dGTP) . This specificity is essential for maintaining the balance of nucleotide pools within the cell and preventing the incorporation of potentially harmful nucleotide analogs into DNA or RNA .

Biological Significance

The activity of DCTPP1 is vital for several cellular processes:

  1. DNA Synthesis and Repair: By regulating the levels of dCTP, DCTPP1 ensures the proper synthesis and repair of DNA. Excess dCTP can lead to overmethylation of CpG islands, which can affect gene expression .
  2. Genotoxic Protection: DCTPP1 helps protect the cell from genotoxic nucleotide analogs by hydrolyzing them, thereby preventing their incorporation into DNA or RNA .
  3. Metabolism of Nucleotides: DCTPP1 is involved in the broader metabolic pathways of nucleotides, contributing to the overall nucleotide homeostasis within the cell .
Genetic and Protein Information

The DCTPP1 gene is located on chromosome 16 at the cytoband p11.2 . It has multiple transcript variants, with at least one being protein-coding . The protein encoded by this gene is intracellular and has been confirmed at the protein level .

Research and Clinical Implications

Research on DCTPP1 has implications for understanding various genetic disorders and diseases related to nucleotide metabolism. The enzyme’s role in eliminating excess dCTP and preventing overmethylation of CpG islands makes it a potential target for therapeutic interventions in conditions where these processes are disrupted .

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