CYTH1 Human

Cytohesin 1 Human Recombinant
Cat. No.
BT27224
Source
E.coli.
Synonyms
Cytohesin 1, D17S811E, PH, SEC7 And Coiled-Coil Domain-Containing Protein 1, Pleckstrin Homology, Sec7 And Coiled-Coil Domains 1, SEC7 Homolog B2-1, PSCD1, SEC7, Homolog Of Secretory Protein SEC7, Cytoadhesin 1, CYTOHESIN-1, B2-1, Cytohesin-1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CYTH1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 421 amino acids (1-398 a.a) and having a molecular mass of 48.8kDa.
CYTH1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Cytohesin 1, also known as CYTH1, is a member of the PSCD family. This protein plays a crucial role in promoting guanine-nucleotide exchange on ARF1 and ARF5, thereby activating these ARF factors by facilitating the replacement of GDP with GTP.
Description
CYTH1 Human Recombinant, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 421 amino acids (specifically, amino acids 1-398). This protein has a molecular mass of 48.8 kDa. Notably, our CYTH1 is fused to a 23 amino acid His-tag at its N-terminus and undergoes purification using proprietary chromatographic techniques.
Physical Appearance
The product is a sterile filtered solution that is colorless.
Formulation
This product consists of a CYTH1 protein solution (0.5 mg/ml) in a buffer containing Phosphate buffered saline (pH 7.4), 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product should be kept at 4°C. For longer storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for extended storage. To maintain product integrity, avoid repeated cycles of freezing and thawing.
Purity
The purity of this product exceeds 85%, as determined by SDS-PAGE analysis.
Synonyms
Cytohesin 1, D17S811E, PH, SEC7 And Coiled-Coil Domain-Containing Protein 1, Pleckstrin Homology, Sec7 And Coiled-Coil Domains 1, SEC7 Homolog B2-1, PSCD1, SEC7, Homolog Of Secretory Protein SEC7, Cytoadhesin 1, CYTOHESIN-1, B2-1, Cytohesin-1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMEEDDSY VPSDLTAEER QELENIRRRK QELLADIQRL KDEIAEVANE IENLGSTEER KNMQRNKQVA MGRKKFNMDP KKGIQFLIEN DLLKNTCEDI AQFLYKGEGL NKTAIGDYLG ERDEFNIQVL HAFVELHEFT DLNLVQALRQ FLWSFRLPGE AQKIDRMMEA FAQRYCQCNN GVFQSTDTCY VLSFAIIMLN TSLHNPNVKD KPTVERFIAM NRGINDGGDL PEELLRNLYE SIKNEPFKIP EDDGNDLTHT FFNPDREGWL LKLGGGRVKT WKRRWFILTD NCLYYFEYTT DKEPRGIIPL ENLSIREVED SKKPNCFELY IPDNKDQVIK ACKTEADGRV VEGNHTVYRI SAPTPEEKEE WIKCIKAAIS RDPFYEMLAA RKKKVSSTKR H.

Product Science Overview

Structure and Expression

Cytohesin 1 is a protein that consists of 398 amino acids and is expressed in various tissues, including hematopoietic stem and progenitor cells (HSPCs). The recombinant form of Cytohesin 1 is typically produced in Escherichia coli and purified to a high degree of purity, making it suitable for various experimental applications .

Function and Mechanism

Cytohesin 1 is primarily involved in the regulation of cell adhesion and migration. It acts as a GEF for ARFs, which are small GTP-binding proteins that regulate vesicular trafficking and actin cytoskeleton dynamics. By activating ARFs, Cytohesin 1 facilitates the interaction of cells with the extracellular matrix and other cells, thereby influencing cell movement and positioning .

In hematopoietic stem and progenitor cells, Cytohesin 1 has been identified as a critical mediator of adhesion to the bone marrow niche. This adhesion is essential for the homing and engraftment of these cells following transplantation. Knockdown studies have shown that the absence of Cytohesin 1 impairs the ability of HSPCs to adhere to fibronectin and ICAM1, which are integrin ligands. This results in reduced integrin β1 activation and subsequently lower long-term engraftment levels .

Clinical Relevance

The role of Cytohesin 1 in cell adhesion and migration makes it a significant protein in the context of hematopoietic stem cell transplantation. Understanding its function can help improve the efficiency of stem cell therapies for treating blood diseases and cancers. Additionally, Cytohesin 1’s involvement in integrin activation and cell adhesion suggests potential therapeutic targets for modulating immune cell functions and treating various inflammatory conditions .

Research and Applications

Recombinant Cytohesin 1 is widely used in research to study its role in cell adhesion, migration, and signal transduction. It is also utilized in experiments aimed at understanding the molecular mechanisms underlying hematopoietic stem cell homing and engraftment. The high purity of recombinant Cytohesin 1 makes it suitable for various biochemical and biophysical assays, including SDS-PAGE and mass spectrometry .

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