CYP2E1 Human
Description
Product Specs
Product Science Overview
Cytochrome P450 2E1 (CYP2E1) is a member of the cytochrome P450 superfamily of enzymes, which play a crucial role in the metabolism of various substances in the body. This enzyme is particularly notable for its involvement in the metabolism of both endogenous and exogenous compounds, including drugs, toxins, and carcinogens .
The CYP2E1 enzyme is encoded by the CYP2E1 gene located on chromosome 10 in humans . The enzyme is a membrane protein predominantly found in the liver, where it constitutes a significant portion of the total hepatic cytochrome P450 content . The recombinant form of this enzyme is often expressed in systems such as Saccharomyces cerevisiae (yeast) for research and industrial purposes .
CYP2E1 is involved in the oxidation of small, polar molecules. It metabolizes a variety of substrates, including ethanol, acetone, and acetal, as well as toxic compounds like benzene, carbon tetrachloride, and nitrosamines . This enzyme is also implicated in the bioactivation of certain carcinogens and toxins, which can lead to hepatotoxicity .
One of the key roles of CYP2E1 is in the metabolism of ethanol. It converts ethanol to acetaldehyde and then to acetate, working alongside alcohol dehydrogenase and aldehyde dehydrogenase . This process is particularly important in conditions of chronic alcohol consumption, where CYP2E1 levels are induced .
CYP2E1 has been associated with various diseases and conditions. Its elevated expression is linked to the production of reactive oxygen species, which can contribute to oxidative stress and tissue damage . This enzyme is also involved in the metabolism of several drugs, making it a critical factor in drug interactions and toxicity .
In addition, CYP2E1 is induced by factors such as ethanol, diabetes, and starvation . Its role in the metabolism of toxic environmental chemicals and carcinogens highlights its importance in studies related to cancer, liver diseases, and other metabolic disorders .
Recombinant CYP2E1 is widely used in research to study its metabolic activity and interactions with various compounds . It is also utilized in drug metabolism and pharmacokinetics research to identify the specificity and activity of this enzyme against different substrates . The recombinant form allows for controlled studies and the development of selective inhibitors that can modulate its activity .
