Cyclophilin A Rat
Cyclophilin-A Rat Recombinant
Cat. No.
BT2927
Source
Escherichia Coli.
Synonyms
Peptidyl-prolyl cis-trans isomerase A, EC:5.2.1.8, PPIase A, Cyclophilin A, Cyclosporin A-binding protein, Rotamase A, p1B15, p31, Ppia.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Cyclophilin-A Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Val2-Leu164) containing 173 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 19kDa.
Product Specs
Introduction
PPIases are a type of enzyme that facilitates the folding of proteins by catalyzing the cis-trans isomerization of proline imidic peptide bonds within oligopeptides. Cyclophilin-A, a cyclosporin binding-protein, might be involved in the immunosuppressive mechanism of cyclosporin A. This protein is also known to interact with various HIV proteins, including p55 gag, Vpr, and capsid protein, playing a crucial role in the formation of infectious HIV virions. Several pseudogenes of Cyclophilin-A have been identified across different chromosomes.
Description
Recombinant Cyclophilin-A from Rat, produced in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 173 amino acids, including a 10 amino acid His tag located at the N-terminus, and encompasses amino acids Val2 to Leu164. The calculated molecular mass of the protein is 19kDa.
Physical Appearance
White powder, lyophilized (freeze-dried) and filtered.
Formulation
Cyclophilin-A was lyophilized from a 0.5mg/ml solution in phosphate buffered saline containing 5% (w/v) trehalose. The solution was filtered through a 0.4μm filter before lyophilization.
Solubility
To prepare a working stock solution, add deionized water to the lyophilized pellet to achieve a concentration of approximately 0.5mg/ml. Allow the pellet to dissolve completely. Note: Cyclophilin-A is not sterile. Before using in cell culture, filter the product through an appropriate sterile filter.
Stability
Store the lyophilized protein at -20°C. After reconstitution, aliquot the product to prevent repeated freezing and thawing cycles. Reconstituted protein remains stable at 4°C for a limited period; no changes were observed after storage at 4°C for two weeks.
Purity
Purity is determined to be greater than 95.0% by SDS-PAGE analysis.
Synonyms
Peptidyl-prolyl cis-trans isomerase A, EC:5.2.1.8, PPIase A, Cyclophilin A, Cyclosporin A-binding protein, Rotamase A, p1B15, p31, Ppia.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHASVNPTVFFDIT ADGEPLGRVC FELFADKVPK TAENFRALST GEKGFGYKGS SFHRIIPGFM CQGGDFTRHN GTGGKSIYGE KFEDENFILK HTGPGILSMA NAGPNTNGSQ FFICTAKTEW LDGKHVVFGK VKEGMSIVEA MERFGSRNGK TSKKITISDC GQL.
Product Science Overview
Introduction
Cyclophilin-A (CypA) is a member of the immunophilin family, which is known for its role in binding to immunosuppressive drugs like cyclosporine A. This protein is highly conserved across species, including humans, mice, and rats. Cyclophilin-A is the most abundantly expressed isozyme among the 18 known cyclophilins in humans .
Structure and Function
Cyclophilin-A is characterized by its peptidyl-prolyl isomerase (PPIase) activity, which catalyzes the cis-trans isomerization of peptide bonds at proline residues. This activity is crucial for protein folding and function . The protein consists of 164 amino acids and has a molecular weight of approximately 18 kDa .
Biological Roles
Cyclophilin-A plays a vital role in various biological processes, including:
- Protein Folding: Facilitates the proper folding of newly synthesized proteins.
- Immune Regulation: Acts as an intracellular receptor for cyclosporine A, modulating immune responses.
- Inflammatory Response: Involved in the regulation of inflammatory processes.
- Cholesterol Metabolism: Participates in the regulation of cholesterol levels in the body .
Clinical Significance
Cyclophilin-A has been implicated in several diseases and conditions:
- Cardiovascular Diseases: Plays a role in the development of atherosclerosis and other cardiovascular conditions.
- Viral Infections: Involved in the replication and infection processes of various viruses, including HIV and hepatitis C .
- Cancer: Overexpression of Cyclophilin-A has been observed in several types of cancer, including breast cancer and osteosarcoma .
- Neurodegenerative Diseases: Associated with the progression of diseases like Alzheimer’s and Parkinson’s .
Recombinant Cyclophilin-A (Rat)
Applications
Recombinant Cyclophilin-A (Rat) is used in various research applications, including:
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
