Cyclophilin A Mouse
Cyclophilin A Mouse Recombinant
Cat. No.
BT2777
Source
E.coli.
Synonyms
Peptidyl-prolyl cis-trans isomerase A, PPIase A, Cyclophilin A, Cyclosporin A-binding protein, Rotamase A, SP18, Peptidyl-prolyl cis-trans isomerase A, N-terminally processed, Ppia, 2700098C05 Cphn, CyP-18, CypA.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
Cyclophilin A Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids (1-164a.a.) and having a molecular mass of 20.4kDa.
Cyclophilin A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Cyclophilin A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Peptidyl-prolyl cis-trans isomerases (PPIases) are a class of enzymes that catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. This isomerization is a rate-limiting step in protein folding, and PPIases accelerate this process. Cyclophilin A (CypA) is a member of the cyclophilin family of PPIases and is the most abundant cyclophilin in humans. CypA is a ubiquitously expressed protein that is found in the cytoplasm and nucleus. It binds to the immunosuppressive drug cyclosporin A (CsA) and is thought to mediate its immunosuppressive effects. CypA has been implicated in a variety of cellular processes, including protein folding, signal transduction, and cell cycle regulation. It has also been shown to interact with a number of viral proteins, including the HIV-1 capsid protein, and may play a role in viral replication.
Description
Recombinant Mouse Cyclophilin A protein was expressed in E. coli. It is a single, non-glycosylated polypeptide chain containing 187 amino acids (1-164a.a.) and having a molecular mass of 20.4 kDa. The protein is fused to a 23 amino acid His-tag at the N-terminus and is purified by proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The protein solution (0.5 mg/ml) is supplied in phosphate-buffered saline (pH 7.4) containing 10% glycerol and 1 mM DTT.
Stability
The protein is stable at 4°C for 2-4 weeks. For long-term storage, it is recommended to store the protein at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE.
Synonyms
Peptidyl-prolyl cis-trans isomerase A, PPIase A, Cyclophilin A, Cyclosporin A-binding protein, Rotamase A, SP18, Peptidyl-prolyl cis-trans isomerase A, N-terminally processed, Ppia, 2700098C05 Cphn, CyP-18, CypA.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMVNPTVF FDITADDEPL GRVSFELFAD KVPKTAENFR ALSTGEKGFG YKGSSFHRII PGFMCQGGDF TRHNGTGGRS IYGEKFEDEN FILKHTGPGI LSMANAGPNT NGSQFFICTA KTEWLDGKHV VFGKVKEGMN IVEAMERFGS RNGKTSKKIT ISDCGQL.
Product Science Overview
Introduction
Cyclophilin A (CypA) is a member of the immunophilin family, which is known for its peptidyl-prolyl isomerase activity. This activity facilitates the cis-trans isomerization of peptide bonds at proline residues, aiding in protein folding. Cyclophilin A is highly conserved across species and is found in various organisms, including humans and mice.
Structure and Expression
Cyclophilin A in mice is a protein consisting of 164 amino acids with a molecular weight of approximately 17 kDa . It is expressed ubiquitously in mammalian cells and is particularly abundant in the cytoplasm. The recombinant form of Cyclophilin A (Mouse) is typically produced in E. coli expression systems and can be tagged or untagged depending on the application .
Biological Functions
Cyclophilin A plays several critical roles in cellular processes:
- Protein Folding: It assists in the proper folding of proteins by catalyzing the isomerization of proline residues.
- Immune Regulation: Cyclophilin A is involved in modulating immune responses. It acts as a chaperone and is implicated in the regulation of inflammatory processes.
- Signal Transduction: It participates in various signaling pathways, influencing cellular responses to external stimuli.
Clinical Significance
Cyclophilin A has been linked to numerous diseases and conditions:
- Cardiovascular Diseases: It is secreted by vascular smooth muscle cells in response to inflammatory stimuli and contributes to atherosclerosis .
- Infectious Diseases: Cyclophilin A interacts with viral proteins, influencing the replication cycle of viruses. It is also involved in bacterial infections by regulating host cell processes .
- Cancer: As a proinflammatory factor and immune regulator, Cyclophilin A has potential applications in cancer immunotherapy .
Recombinant Cyclophilin A Applications
Recombinant Cyclophilin A (Mouse) is used in various research and clinical applications:
- Drug Development: It serves as a target for developing inhibitors that can modulate its activity in diseases.
- Biotherapeutics: Recombinant Cyclophilin A is explored for its potential in treating conditions like cancer, brain pathologies, and as a supportive treatment in cyclosporine A-based therapies .
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