CXCL16 Mouse

Mouse CXCL16 is a CXC chemokine with a transmembrane domain and lacks the ELR motif. The cDNA of mouse CXCL16 encodes a precursor protein of 246 amino acids, comprising a predicted 26-amino acid signal peptide, an 88-amino acid chemokine domain, an 87-amino acid mucin-like spacer region, a 22-amino acid transmembrane domain, and a 23-amino acid cytoplasmic tail. This chemokine elicits a significant chemotactic response and calcium mobilization. Moreover, it serves as a scavenger receptor on macrophages, exhibiting specific binding to oxidized low-density lipoprotein (OxLDL), suggesting a potential involvement in atherogenesis. Mouse CXCL16 is produced by dendritic cells in lymphoid organ T cell zones and by cells in the splenic red pulp, existing in both membrane-bound and soluble forms. Its receptor, CXCR6/Bonzo (STRL33 and TYMSTR), mediates its effects. CXCL16 is predominantly found in the spleen, lymph nodes, and Peyer's patches. It is also expressed in non-lymphoid tissues, including the lung, kidney, small intestine, and thymus, with low expression in the heart and liver and no detectable expression in the brain, purified B cells, or T cells. CXCL16 deficiency is implicated in breast cancer progression. It also plays a role in immune-mediated liver injury by regulating T lymphocyte infiltration in the liver. Additionally, CXCL16 has a distinct function in maintaining cardiac allograft tolerance through natural killer T (NKT) cells. It is crucial not only for IFN-gamma production by NKT cells but also for promoting Th1-biased immune responses mediated by these cells.

Recombinant Mouse CXCL16, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 88 amino acids with a molecular weight of 9.9 kDa. The purification of CXCL16 is achieved using proprietary chromatographic methods.

White, lyophilized (freeze-dried) powder, sterile.

The protein was lyophilized from a solution at a concentration of 1.0 mg/ml in a buffer containing 20 mM phosphate buffer (PB), pH 7.4, and 50 mM NaCl.

To reconstitute the lyophilized Mouse CXCL16, it is recommended to dissolve it in sterile 18MΩ-cm H2O at a minimum concentration of 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.

Lyophilized CXCL16 remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated at a temperature below -18°C. After reconstitution, CXCL16 should be stored at 4°C for 2-7 days. For future use, it should be stored below -18°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.

The purity is determined to be greater than 97.0% using the following methods: (a) RP-HPLC analysis and (b) SDS-PAGE analysis.

The biological activity is assessed by the ability to induce chemotaxis in murine lymphocytes within a concentration range of 100-1000 ng/ml, corresponding to a specific activity of 1,000-10,000 IU/mg.

C-X-C motif chemokine 16, Small-inducible cytokine B16, Transmembrane chemokine CXCL16, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, SR-PSOX, Cxcl16, Srpsox, Zmynd15, AV290116, BB024863, 0910001K24Rik.

Escherichia Coli.

NQGSVAGSCS CDRTISSGTQ IPQGTLDHIR KYLKAFHRCP FFIRFQLQSK SVCGGSQDQW VRELVDCFER KECGTGHGKS FHHQKHLP.