BRAK Rat
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
The CXCL14 is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
The CXCL14 gene encodes a precursor protein consisting of 99 amino acids, which includes a 22 amino acid signal peptide that is cleaved to produce the mature 77 amino acid protein . The mature protein has a calculated molecular weight of approximately 9.4 kDa . Four conserved cysteines in the mature protein form two intrachain disulfide bonds, which are crucial for its structural stability .
CXCL14 is expressed in a wide range of tissues, including the breast, kidney, and other normal tissues . It is known for its chemotactic activity, particularly for monocytes, but not for B and T cells .
CXCL14 plays a significant role in various biological processes:
- Chemotaxis: CXCL14 exhibits chemotactic activity for monocytes and monocyte-derived dendritic cells and natural killer cells . This makes it an important player in the immune response.
- Tumor Suppression: Recent studies have highlighted the role of CXCL14 in tumor suppression. It has been shown to inhibit tumorigenesis and motility, making it a potential therapeutic candidate for cancer treatment .
- Metabolism: CXCL14 is also involved in fat metabolism modulation, which could have implications for obesity and related metabolic disorders .
Recombinant CXCL14 can be produced in various expression systems, with Escherichia coli being a common choice due to its simplicity and cost-effectiveness . The recombinant protein is typically purified using techniques such as nickel affinity chromatography and ion exchange chromatography to achieve high purity and bioactivity . The endotoxin level in the purified protein is kept low to ensure its safety for in vivo studies .
Recombinant CXCL14 has several applications in research and therapeutic development:
- Cancer Research: Due to its tumor-suppressive properties, CXCL14 is being studied as a potential therapeutic agent for cancer treatment.
- Immunology: Its role in chemotaxis makes it a valuable tool for studying immune cell migration and function.
- Metabolic Studies: CXCL14’s involvement in fat metabolism makes it a candidate for research into obesity and metabolic disorders.
In summary, BRAK (CXCL14) (Rat Recombinant) is a versatile and biologically significant chemokine with potential applications in cancer therapy, immunology, and metabolic research. Its recombinant production allows for extensive study and potential therapeutic use.
