BRAK Human, His
BRAK Human Recombinant (CXCL14), His-Tag
Cat. No.
BT9605
Source
Escherichia Coli.
Synonyms
C-X-C motif chemokine 14, Small-inducible cytokine B14, Chemokine BRAK, Bolekine, NJAC, KS1, Kec, BMAC, MIP-2g, SCYB14, CXCL14, BRAK, MGC10687.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
CXCL14 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 88 amino acids and having a molecular mass of 10.66 kDa.
The Human BRAK contains a 10 a.a. fusion His tag at N-Terminus.
The BRAK is purified by proprietary chromatographic techniques.
The Human BRAK contains a 10 a.a. fusion His tag at N-Terminus.
The BRAK is purified by proprietary chromatographic techniques.
Product Specs
Introduction
CXCL14, also known as BRAK, is a chemokine that plays a role in immune regulation and inflammation. It exhibits chemotactic activity for monocytes, but not for other immune cells like lymphocytes, dendritic cells, neutrophils, or macrophages. CXCL14 contributes to the maintenance of monocyte-derived macrophages.
Description
Recombinant Human CXCL14, expressed in E. coli, is a single, non-glycosylated polypeptide chain with a molecular weight of 10.66 kDa. It comprises 88 amino acids and includes a 10-amino acid His tag at the N-terminus. Purification of BRAK is achieved through proprietary chromatographic techniques.
Physical Appearance
White, lyophilized powder.
Formulation
CXCL14 is supplied as a lyophilized powder, filtered through a 0.4 μm filter. The protein was initially concentrated to 0.5 mg/ml in a 20 mM Tris buffer with 20 mM NaCl at pH 7.5 before lyophilization.
Solubility
To reconstitute lyophilized CXCL14, it is recommended to dissolve it in sterile 18 MΩ-cm H2O to a concentration of at least 100 μg/ml. The solution can be further diluted in other aqueous solutions as needed. Note: This product is not sterile. Before using in cell culture, it must be filtered through an appropriate sterile filter.
Stability
Lyophilized BRAK is stable at room temperature for up to 3 weeks. However, for long-term storage, it should be stored in a dry environment below -18°C. After reconstitution, BRAK can be stored at 4°C for 2-7 days. For extended storage, it is recommended to add a carrier protein like HSA or BSA (0.1%). Avoid repeated freeze-thaw cycles.
Purity
The purity of CXCL14 is greater than 95.0%, as determined by RP-HPLC and SDS-PAGE analysis.
Synonyms
C-X-C motif chemokine 14, Small-inducible cytokine B14, Chemokine BRAK, Bolekine, NJAC, KS1, Kec, BMAC, MIP-2g, SCYB14, CXCL14, BRAK, MGC10687.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHAS SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIITTKS VSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE.
Product Science Overview
Classification and Structure
Biological Properties and Functions
CXCL14 exhibits several important biological properties:
- Chemotactic Activity: It displays chemotactic activity for monocytes but not for B and T cells, dendritic cells, neutrophils, or macrophages . This suggests a role in the homeostasis of monocyte-derived macrophages rather than in inflammation.
- Antimicrobial Activity: CXCL14 has been shown to exhibit antimicrobial activity, which is attributed to its amphipathic cationic alpha-helical region in the C-terminus .
- Role in Cancer: CXCL14 is highly expressed in tumor-associated stromal cells and has been implicated in promoting tumor cell growth and invasion . It is also involved in the regulation of epithelial cell proliferation and migration .
Mode of Action
Regulatory Mechanisms
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