CUEDC2 Human

CUEDC2 is a single, non-glycosylated polypeptide chain consisting of 310 amino acids, with a molecular mass of approximately 34 kDa . The recombinant form of this protein is typically produced in Escherichia coli (E. coli) and is fused to a 23 amino acid His-tag at the N-terminus to facilitate purification . The protein is purified using proprietary chromatographic techniques to ensure high purity, typically greater than 90% as determined by SDS-PAGE .

CUEDC2 is known to down-regulate ESR1 (Estrogen Receptor 1) protein levels through the ubiquitination-proteasome pathway . This pathway is crucial for maintaining protein homeostasis within the cell by targeting misfolded or damaged proteins for degradation. Additionally, CUEDC2 controls PGR (Progesterone Receptor) protein levels through a parallel mechanism . These regulatory functions highlight the importance of CUEDC2 in cellular signaling and hormone regulation.

The recombinant form of CUEDC2 is used extensively in research to study its role in cellular processes and its potential implications in diseases. For instance, understanding how CUEDC2 regulates hormone receptors can provide insights into hormone-related cancers and other disorders. The availability of high-purity recombinant CUEDC2 allows researchers to conduct detailed biochemical and structural studies, facilitating the development of targeted therapies.

CUEDC2 is typically stored at 4°C if it is to be used within 2-4 weeks . For longer-term storage, it is recommended to freeze the protein at -20°C, with the addition of a carrier protein such as 0.1% HSA (Human Serum Albumin) or BSA (Bovine Serum Albumin) to prevent degradation . It is important to avoid multiple freeze-thaw cycles to maintain the protein’s stability and functionality .