CTDSPL Human

CTD Small Phosphatase-Like Human Recombinant
Cat. No.
BT27266
Source
Escherichia Coli.
Synonyms
CTD small phosphatase-like protein, CTDSP-like, Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3, NIF-like protein, Nuclear LIM interactor-interacting factor 1, NLI-interacting factor 1, Protein YA22, hYA22, RBSP3, Small C-terminal domain phosphatase 3, SCP3, Small CTD phosphatase 3, CTDSPL, C3orf8, NIF1, NIFL, SCP3, YA22, PSR1.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

CTDSPL Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 209 amino acids (82-265) and having a molecular mass of 23.9kDa.
CTDSPL is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
CTD small phosphatase-like protein (CTDSPL) is responsible for the dephosphorylation of the 'Ser-5' residue within the repeating heptad sequence found in the C-terminal domain (CTD) of RNA polymerase II's largest subunit, POLR2A. This process is crucial for regulating transcription by RNA polymerase II. CTDSPL likely facilitates the shift from transcription initiation and capping to the elongation of the transcript. In essence, CTDSPL acts as a negative regulator of RNA polymerase II transcription. This protein is utilized by the REST complex to silence neuronal genes containing RE-1 elements in non-neuronal cells.
Description
Recombinant human CTDSPL, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein consists of 209 amino acids (residues 82-265), resulting in a molecular weight of 23.9 kDa. For purification purposes, a 24 amino acid His-tag is fused to the N-terminus of the CTDSPL protein. Proprietary chromatographic methods ensure a high level of purity.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The CTDSPL protein is supplied at a concentration of 0.5 mg/ml in a buffer solution containing 20mM Tris-HCl (pH 8.0), 40% glycerol, 0.15M NaCl, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the CTDSPL solution should be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To maintain stability during long-term storage, consider adding a carrier protein (0.1% HSA or BSA). It's important to avoid repeated freeze-thaw cycles.
Purity
Purity of the CTDSPL protein is determined to be greater than 90.0% by SDS-PAGE analysis.
Synonyms
CTD small phosphatase-like protein, CTDSP-like, Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3, NIF-like protein, Nuclear LIM interactor-interacting factor 1, NLI-interacting factor 1, Protein YA22, hYA22, RBSP3, Small C-terminal domain phosphatase 3, SCP3, Small CTD phosphatase 3, CTDSPL, C3orf8, NIF1, NIFL, SCP3, YA22, PSR1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMKYLLP EVTVLDYGKK CVVIDLDETL VHSSFKPISN ADFIVPVEID GTIHQVYVLK RPHVDEFLQR MGQLFECVLF TASLAKYADP VADLLDRWGV FRARLFRESC VFHRGNYVKD LSRLGRELSK VIIVDNSPAS YIFHPENAVP VQSWFDDMTD TELLDLIPFF EGLSREDDVY SMLHRLCNR.

Product Science Overview

Gene and Protein Structure

The CTDSPL gene is a protein-coding gene associated with various biological processes, including phosphatase activity and phosphoprotein phosphatase activity . The recombinant form of this protein, often tagged with a His-tag for purification purposes, is produced in E. coli and corresponds to the amino acids 82-265 of the human CTDSPL .

Biological Function

CTDSPL plays a crucial role in the regulation of gene expression by dephosphorylating the RNA polymerase II CTD. This dephosphorylation is essential for the transition from transcription initiation to elongation and for the recycling of RNA polymerase II . The protein’s activity is vital for proper transcriptional regulation and cellular function.

Clinical Significance

Research has shown that CTDSPL can influence the expression of certain genes. For instance, CTDSPL2, a paralog of CTDSPL, has been found to increase the expression of ε- and γ-globin genes in K562 cells and CD34+ cells derived from umbilical cord blood . This finding suggests potential therapeutic applications for CTDSPL in treating conditions like sickle cell disease and β-thalassemia by reactivating fetal hemoglobin production .

Recombinant Protein Production

Recombinant human CTDSPL is produced using bacterial expression systems, such as E. coli. The protein is typically purified using affinity chromatography, leveraging the His-tag for efficient purification . The recombinant protein is used in various research applications to study its function and potential therapeutic uses.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

CTDSP1 Human
CTD Small Phosphatase 1 Human Recombinant
Cat. No.
BT27194
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.