CTBP1 Human

C-Terminal Binding Protein 1 Human Recombinant
Cat. No.
BT3212
Source
Escherichia Coli.
Synonyms
EC 1.1.1, BARS, MGC104684, CTBP1, C-terminal-binding protein 1, CTBP.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

CTBP1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 440 amino acids (1-440 a.a.) and having a molecular mass of 47.5kDa.
CTBP1 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
CTBP1 is a protein involved in various cellular processes. It helps regulate the structure of the Golgi complex, a cellular organelle. CTBP1 also plays a role in the differentiation of brown adipose tissue, a type of fat tissue involved in energy expenditure. Functionally, CTBP1 exhibits dehydrogenase activity and interacts with other proteins, such as adenovirus E1A proteins and a polycomb group protein complex. These interactions suggest CTBP1's involvement in transcriptional repression and the regulation of gene expression during development.
Description
This product consists of the CTBP1 protein, produced in E. coli bacteria. It is a single polypeptide chain of 440 amino acids, lacking any glycosylation modifications. With a molecular weight of 47.5 kDa, the protein is purified using specialized chromatographic methods, ensuring its high quality and purity.
Physical Appearance
The product appears as a clear, colorless solution that has been sterilized by filtration.
Formulation
The CTBP1 protein is supplied in a solution containing 20mM Tris-HCl buffer at pH 8, 100mM NaCl (sodium chloride), and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), keep the product refrigerated at 4°C. For extended storage, freeze the solution at -20°C. To ensure optimal protein stability during long-term storage, adding a carrier protein like HSA or BSA (0.1%) is recommended. Repeated freezing and thawing cycles should be minimized to maintain product integrity.
Purity
The purity of the CTBP1 protein in this product exceeds 90%, as determined by SDS-PAGE analysis, a widely used technique for protein separation and purity assessment.
Synonyms
EC 1.1.1, BARS, MGC104684, CTBP1, C-terminal-binding protein 1, CTBP.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHLLNKG LPLGVRPPIM NGPLHPRPLV ALLDGRDCTV EMPILKDVAT VAFCDAQSTQ EIHEKVLNEA VGALMYHTIT LTREDLEKFK ALRIIVRIGS GFDNIDIKSA GDLGIAVCNV PAASVEETAD STLCHILNLY RRATWLHQAL REGTRVQSVE QIREVASGAA RIRGETLGII GLGRVGQAVA LRAKAFGFNV LFYDPYLSDG VERALGLQRV STLQDLLFHS DCVTLHCGLN EHNHHLINDF TVKQMRQGAF LVNTARGGLV DEKALAQALK EGRIRGAALD VHESEPFSFS QGPLKDAPNL ICTPHAAWYS EQASIEMREE AAREIRRAIT GRIPDSLKNC VNKDHLTAAT HWASMDPAVV HPELNGAAYR YPPGVVGVAP TGIPAAVEGI VPSAMSLSHG LPPVAHPPHA PSPGQTVKPE ADRDHASDQL.

Product Science Overview

Introduction

C-Terminal Binding Protein 1 (CtBP1) is a multifunctional protein that plays a significant role in various cellular processes, including transcriptional regulation, cell proliferation, and apoptosis. It is encoded by the CTBP1 gene in humans and is known for its ability to bind to the C-terminus of adenovirus E1A proteins .

Discovery and Structure

CtBP1 was initially identified as a protein that interacts with the C-terminal portion of adenovirus E1A, a protein involved in the transcriptional regulation of key cellular genes . Structurally, CtBP1 belongs to the hydroxyacid-dehydrogenase family and possesses a NAD(H)-binding Rossmann fold, which controls its oligomerization and cellular functions .

Functions and Mechanisms

CtBP1 functions primarily as a transcriptional co-repressor. It binds to sequence-specific DNA-binding proteins and helps turn genes off . This protein and its closely related counterpart, CtBP2, can dimerize and act as transcriptional repressors, playing a role in cellular proliferation .

CtBP1 is involved in various biological processes, including:

  • Transcriptional Regulation: CtBP1 acts as a transcriptional co-repressor, regulating gene networks associated with cancer hallmarks and malignant behavior .
  • Cell Proliferation and Survival: It has pro-tumorigenic functions, promoting cell survival, proliferation, and migration/invasion .
  • Membrane Trafficking and Mitosis: CtBP1 is required for membrane trafficking and Golgi complex partitioning during mitosis .
Clinical Significance

CtBP1 overexpression has been observed in multiple cancers, where it contributes to pro-tumorigenic functions . Targeting CtBP1 with selective inhibitors has shown potential in hindering its cellular functions, making it a promising target for cancer therapy .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

CTBP1 Antibody
C-Terminal Binding Protein 1, Monoclonal Mouse Anti Human Antibody
Cat. No.
BT16103
Source
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.