CTACK Mouse

Mature mouse CCL27 is a 95 amino acid protein that shares 57% amino acid sequence identity with human CCL27 and 87% with rat CCL27 . The recombinant form of this protein is often produced in E. coli and has a predicted molecular mass of approximately 10.9 kDa .

CCL27 is primarily expressed by keratinocytes, the predominant cell type in the epidermis. It is constitutively expressed, meaning it is always present, but its expression can be upregulated by inflammatory stimuli and in wounded skin . This chemokine is involved in attracting T cells to the skin, which is essential for immune surveillance and response to pathogens.

An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration . This indicates that CCL27 has multiple roles in cellular processes beyond its chemotactic activity.

The activity of recombinant mouse CCL27 is measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with mouse CCR10. The effective dose (ED50) for this activity is typically in the range of 0.05-0.25 µg/mL . This chemotactic activity is crucial for the recruitment of immune cells to sites of inflammation or injury.

Recombinant mouse CCL27 is widely used in research to study immune responses, particularly in the context of skin diseases and wound healing. It is available in both carrier-free and carrier-containing formulations, depending on the specific requirements of the experimental setup .