CRHBP Human

Corticotropin Releasing Hormone Binding Protein Human Recombinant
Cat. No.
BT21785
Source
Escherichia Coli.
Synonyms
Corticotropin releasing hormone binding protein, CRF-BP, CRH-BP, CRF-binding protein.
Appearance
Filtered white lyophilized powder.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CRHBP Human Recombinant is a 34.58 kDa protein containing 308 aa and fused to a 10 aa N-Terminal His-tag.
CRHBP is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Corticotropin-releasing hormone (CRH) is a key regulator of the synthesis and secretion of peptides derived from proopiomelanocortin. In humans, CRH levels in peripheral circulation are typically low. These levels increase during pregnancy and rapidly decline after childbirth. The placenta is believed to be the primary source of maternal plasma CRH. Human plasma contains a CRH-binding protein (CRHBP) that inactivates CRH, potentially preventing excessive stimulation of the pituitary-adrenal axis during pregnancy.
Description
Recombinant human CRHBP is a 34.58 kDa protein consisting of 308 amino acids. It includes a 10 amino acid N-terminal His-tag and is purified using proprietary chromatographic methods.
Physical Appearance
White lyophilized powder following filtration.
Formulation
Human CRHBP undergoes filtration (0.4 μm) and lyophilization from a 0.5 mg/ml solution in 20 mM TRIS, 20 mM NaCl, pH 7.5.
Solubility
To create a working stock solution of about 0.5 mg/ml, add deionized water to the lyophilized pellet and allow it to dissolve completely. Please note that this product is not sterile. Before using in cell culture, filter the product through a sterile filter of appropriate pore size.
Stability
The lyophilized protein should be stored at -20°C. To minimize repeated freeze-thaw cycles, aliquot the reconstituted protein and store at -80°C for extended periods. Reconstituted protein remains stable at 4°C for a limited time and shows no significant changes after one week at this temperature.
Applications
Suitable applications include enzyme-linked immunosorbent assay (ELISA) and Western blotting.
Synonyms
Corticotropin releasing hormone binding protein, CRF-BP, CRH-BP, CRF-binding protein.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHAS YLELREAADY DPFLLFSANL KRELAGEQPY RRALRCLDML SLQGQFTFTA DRPQLHCAAF FISEPEEFIT IHYDQVSIDC QGGDFLKVFD GWILKGEKFP SSQDHPLPSA ERYIDFCESG LSRRSIRSSQ NVAMIFFRVH EPGNGFTLTI KTDPNLFPCN VISQTPNGKF TLVVPHQHRN CSFSIIYPVV IKISDLTLGH VNGLQLKKSS AGCEGIGDFV ELLGGTGLDP SKMTPLADLC YPFHGPAQMK VGCDNTVVRM VSSGKHVNRV TFEYRQLEPY ELENPNGNSI GEFCLSGL YLELREAADY DPFLLFSANL KRELAGEQPY RRALRCLDML SLQGQFTFTA DRPQLHCAAF FISEPEEFIT IHYDQVSIDC QGGDFLKVFD GWILKGEKFP SSQDHPLPSA ERYIDFCESG LSRRSIRSSQ NVAMIFFRVH EPGNGFTLTI KTDPNLFPCN VISQTPNGKF TLVVPHQHRN CSFSIIYPVV IKISDLTLGH VNGLQLKKSS AGCEGIGDFV ELLGGTGLDP SKMTPLADLC YPFHGPAQMK VGCDNTVVRM VSSGKHVNRV TFEYRQLEPY ELENPNGNSI GEFCLSGL

Product Science Overview

Introduction

Corticotropin Releasing Hormone Binding Protein (CRHBP) is a crucial component in the regulation of the hypothalamic-pituitary-adrenal (HPA) axis. This protein binds to Corticotropin Releasing Hormone (CRH), regulating its bioavailability and thus playing a significant role in the body’s response to stress .

Structure and Function

CRHBP is a 37 kDa secreted glycoprotein that binds both CRH and urocortin with high affinity . It is structurally unrelated to the CRH receptors, which are responsible for mediating the effects of CRH. The binding of CRHBP to CRH prevents CRH from interacting with its receptors, thereby modulating the activity of the HPA axis .

Biological Role

CRHBP plays a pivotal role in the adjustment of neuroendocrine, autonomic, and behavioral adaptations to stress, ultimately leading to the release of cortisol . This regulation is essential for maintaining homeostasis during stressful conditions. Additionally, CRHBP has been implicated in various physiological processes, including pregnancy, where it prevents inappropriate pituitary-adrenal stimulation .

Clinical Significance

Recent studies have highlighted the importance of CRHBP in hematopoietic function and its potential role as a tumor suppressor in acute myeloid leukemia (AML) . Aberrant hypermethylation and silencing of the CRHBP gene have been observed in a significant number of AML cases, suggesting that loss of CRHBP expression may contribute to the malignant transformation of hematopoietic cells .

Recombinant CRHBP

Human recombinant CRHBP is produced using recombinant DNA technology, which involves inserting the CRHBP gene into a suitable expression system, such as bacteria or mammalian cells. This allows for the large-scale production of CRHBP for research and therapeutic purposes. Recombinant CRHBP retains the same biological activity as the naturally occurring protein, making it a valuable tool for studying the regulation of the HPA axis and its associated disorders.

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Corticotropin Releasing Hormone Binding Protein (22-322 a.a.) Human Recombinant
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Source
<p><span lang="EN-US">Escherichia Coli.</span></p>
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