CRHBP (22-322), Human
Escherichia Coli.
Corticotropin releasing hormone binding protein, CRF-BP, CRH-BP, CRF-binding protein.
Greater than 85.0% as determined by SDS-PAGE.
Description
CRHBP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 324 amino acids (22-322 a.a) and having a molecular mass of 36.1kDa.
CRHBP is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Corticotropin releasing hormone binding protein, CRF-BP, CRH-BP, CRF-binding protein.
Escherichia Coli.
MGSSHHHHHH SSGLVPRGSH MGSESRYLEL REAADYDPFL LFSANLKREL AGEQPYRRAL RCLDMLSLQG QFTFTADRPQ LHCAAFFISE PEEFITIHYD QVSIDCQGGD FLKVFDGWIL KGEKFPSSQD HPLPSAERYI DFCESGLSRR SIRSSQNVAM IFFRVHEPGN GFTLTIKTDP NLFPCNVISQ TPNGKFTLVV PHQHRNCSFS IIYPVVIKIS DLTLGHVNGL QLKKSSAGCE GIGDFVELLG GTGLDPSKMT PLADLCYPFH GPAQMKVGCD NTVVRMVSSG KHVNRVTFEY RQLEPYELEN PNGNSIGEFC LSGL
Product Science Overview
Corticotropin Releasing Hormone Binding Protein (CRHBP) is a crucial protein involved in the regulation of the hypothalamic-pituitary-adrenal (HPA) axis. This protein binds to Corticotropin Releasing Hormone (CRH), modulating its bioavailability and activity. The recombinant form of CRHBP, specifically the 22-322 amino acid sequence, is a significant tool in research and therapeutic applications.
CRHBP is a protein coding gene that plays a pivotal role in the HPA axis by binding to CRH. This binding inactivates CRH, preventing inappropriate pituitary-adrenal stimulation, especially during pregnancy . The protein’s structure allows it to interact with CRH effectively, ensuring the proper regulation of stress responses and other physiological processes.
CRHBP is essential in maintaining the balance of CRH levels in the body. CRH is a potent stimulator of the synthesis and secretion of adrenocorticotropic hormone (ACTH) from the pituitary gland. By binding to CRH, CRHBP regulates its availability, thus controlling the downstream effects on cortisol release and other stress-related hormones .
The dysregulation of CRHBP has been associated with various diseases and conditions. For instance, aberrant hypermethylation and silencing of the CRHBP gene have been observed in acute myeloid leukemia (AML), suggesting its role as a tumor suppressor . Additionally, changes in CRHBP levels have been linked to conditions such as muscular dystrophy-dystroglycanopathy and primary hyperaldosteronism .
The recombinant form of CRHBP, particularly the 22-322 amino acid sequence, is used extensively in research to study its interactions with CRH and its role in various physiological and pathological processes. This recombinant protein is produced using advanced biotechnological methods, ensuring high purity and activity for experimental applications.
