COX5B Human

Cytochrome C Oxidase Subunit Vb Human Recombinant

Cat. No.

BT26365

Source

Escherichia Coli.

Synonyms

Cytochrome c oxidase subunit 5B, mitochondrial precursor, COXVB, COX5B, Mitochondrial, Cytochrome c oxidase polypeptide Vb.

Appearance

Sterile filtered colorless solution.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

COX5B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 121 amino acids (32-129a.a) and having a molecular mass of 13kDa.
COX5B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Cytochrome c oxidase subunit VB (COX5B) is an integral component of the mitochondrial respiratory chain, serving as the terminal enzyme. This multi-subunit enzyme complex facilitates the transfer of electrons from cytochrome c to molecular oxygen, contributing to the generation of a proton electrochemical gradient across the inner mitochondrial membrane. COX5 exists in two isoforms: COX5a and COX5b. The transcription of COX5A, the aerobic isoform, is elevated in response to increased cellular respiration rates and high intracellular oxygen levels. Conversely, under conditions of low oxygen levels, the transcription of COX5B, the hypoxic isoform, is upregulated to optimize the turnover rate of the COX apoenzyme.

Description

Recombinant human COX5B, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 121 amino acids (32-129a.a). With a molecular mass of 13 kDa, COX5B is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.

Physical Appearance

Clear, colorless solution, sterile-filtered.

Formulation

COX5B protein solution at a concentration of 0.5 mg/ml in a buffer composed of 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, 20% glycerol, and 1 mM DTT.

Stability

For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability, the addition of a carrier protein (0.1% HSA or BSA) is advised. Repeated freeze-thaw cycles should be avoided.

Purity

The purity of the COX5B protein is greater than 95.0% as determined by SDS-PAGE analysis.

Synonyms

Cytochrome c oxidase subunit 5B, mitochondrial precursor, COXVB, COX5B, Mitochondrial, Cytochrome c oxidase polypeptide Vb.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSASGGGVP TDEEQATGLE REIMLAAKKG LDPYNVLAPK GASGTREDPN LVPSISNKRI VGCICEEDNT SVVWFWLHKG EAQRCPRCGA HYKLVPQQLA H.

Product Science Overview

Structure and Composition

COX5B is a nuclear-encoded subunit of the cytochrome c oxidase complex. The enzyme complex itself consists of 13 subunits, with COX5B being one of the smaller subunits. The protein is composed of 129 amino acids and has a molecular weight of approximately 14 kDa. The sequence of COX5B is highly conserved and includes three conserved cysteines that coordinate a zinc ion, which is essential for its structural integrity.

Gene and Expression

The COX5B gene is located on the q arm of chromosome 2 at position 11.2 in humans. It is composed of four exons and spans approximately 2,137 base pairs. The gene is expressed in various tissues, with high expression levels observed in the heart, skeletal muscle, and brain.

Function

Cytochrome c oxidase (COX) is responsible for the transfer of electrons from cytochrome c to molecular oxygen, a process that is coupled with the pumping of protons across the inner mitochondrial membrane. This proton gradient drives the synthesis of ATP, the primary energy currency of the cell. COX5B, specifically, is believed to play a regulatory role in the assembly and activity of the cytochrome c oxidase complex.

Clinical Significance

Mutations or deficiencies in COX5B can lead to mitochondrial diseases, which are often characterized by impaired energy production and a wide range of clinical symptoms. These can include muscle weakness, neurodegenerative disorders, and metabolic abnormalities.

Research and Applications

Recombinant COX5B is used in various research applications to study its structure, function, and role in mitochondrial diseases. It is also utilized in the development of therapeutic strategies aimed at correcting mitochondrial dysfunction.

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COX5B Human

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