CoV-2 S1 (16-685), Fc
HEK293 Cells.
Protein is >95% pure as determined SDS-PAGE.
Description
The HEK293 derived recombinant protein contains the Coronavirus 2019 CoV-2 Spike Glycoprotein S1, Wuhan-Hu-1 strain, amino acids 16-685 fused to Fc tag at C-terminal.
Product Specs
The 2019 novel coronavirus (2019-nCoV), a human-infecting coronavirus responsible for causing viral pneumonia, emerged in December 2019 in a fish market in Wuhan, Hubei province, China.
Genetic analysis reveals that 2019-nCoV shares 87% of its identity with the bat-derived severe acute respiratory syndrome coronavirus 2018 (SARS-CoV-2) discovered in Zhoushan, eastern China. Despite some structural differences, the receptor-binding domain (RBD) of 2019-nCoV is similar to that of 2018 SARS-CoV, suggesting a potential binding affinity for the human angiotensin-converting enzyme 2 (ACE2) receptor protein.
While bats are considered the likely reservoir host of 2019-nCoV, researchers hypothesize that an intermediary animal host, potentially from the seafood market, played a role in its transmission to humans. Recombination analysis indicates that the spike glycoprotein of 2019-nCoV is a product of recombination between a bat coronavirus and an unidentified coronavirus.
This recombinant protein, expressed in HEK293 cells, encompasses amino acids 16 to 685 of the Spike Glycoprotein S1 subunit from the Wuhan-Hu-1 strain of Coronavirus 2019 (CoV-2). It is fused with an Fc tag at the C-terminus.
The CoV-2 S1 protein is supplied in a buffer containing sodium chloride, glycine, arginine, adjusted to a pH of 7.5, and supplemented with 5% trehalose.
Lyophilized CoV-2 Spike S1 Glycoprotein exhibits stability at room temperature for up to 3 weeks; however, it is recommended to store it desiccated at a temperature below -18°C. Once reconstituted, the CoV2 Spike protein can be stored at 4°C for 2 to 7 days. For extended storage, it is advisable to store it below -18°C. Adding a carrier protein, such as 0.1% HSA or BSA, is recommended for long-term storage. Repeated freeze-thaw cycles should be avoided.
The purity of the protein exceeds 95%, as determined by SDS-PAGE analysis.
HEK293 Cells.
Purified by Protein-G chromatographic technique.
Product Science Overview
The Coronavirus 2019 Spike Glycoprotein-S1 (16-685 a.a.), Fc Recombinant, is a crucial component in the study of SARS-CoV-2, the virus responsible for the COVID-19 pandemic. This recombinant protein is a fragment of the spike (S) glycoprotein, specifically the S1 subunit, which plays a vital role in the virus’s ability to infect host cells.
The spike glycoprotein of SARS-CoV-2 is a large, trimeric protein that protrudes from the viral surface. It is composed of two subunits: S1 and S2. The S1 subunit, which spans amino acids 16 to 685, contains the receptor-binding domain (RBD) that directly interacts with the host cell receptor, angiotensin-converting enzyme 2 (ACE2). This interaction is critical for viral entry into host cells .
The Fc region in the recombinant protein refers to the fragment crystallizable region of an antibody, which is often fused to other proteins to enhance their stability and facilitate purification. In this context, the Fc fusion helps in the production and purification of the spike glycoprotein-S1 fragment.
The recombinant spike glycoprotein-S1 is extensively used in research to understand the mechanisms of viral entry, immune response, and vaccine development. Studies have shown that the spike S1 domain can act as a TLR4 agonist, upregulating ACE2 expression and inducing a pro-inflammatory response . This makes it a valuable tool for studying the pathogenesis of COVID-19 and the body’s immune response to the virus.
- Vaccine Development: The spike glycoprotein-S1 is a primary target for vaccine development. By understanding how this protein interacts with the host immune system, researchers can design vaccines that elicit a strong and protective immune response.
- Therapeutic Research: The recombinant protein is used to screen for potential therapeutic agents that can block the interaction between the spike protein and ACE2, thereby preventing viral entry.
- Diagnostic Tools: It is also used in the development of diagnostic assays to detect antibodies against SARS-CoV-2 in patient samples, which is crucial for understanding the spread of the virus and immunity in the population.
