SARS MERS Spike S1

Mouse Anti SARS MERS Spike S1
Cat. No.
BT9550
Source
Synonyms
Appearance
Sterile Filtered liquid formulation.
Purity

Greater than 90% as determined by SDS-PAGE gel.

Usage
Prospec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SARS MERS Spike-S1 antibody is specific for the S1 domain of the spike protein of the MERS virus.

Product Specs

Introduction
The SARS coronavirus is characterized by its envelope, which contains three key structural proteins: membrane (M), envelope (E), and spike (S) proteins. The spike (S) glycoprotein plays a crucial role in the virus's ability to infect cells. It interacts with a cellular receptor, facilitating membrane fusion and enabling the virus to enter the target cell. Due to its critical role in the infection cycle, the S-protein is a primary target for neutralizing antibodies. Research has identified SARS as a human coronavirus. Human coronaviruses are a significant cause of upper respiratory tract infections, including the common cold. These viruses are known for having positive-stranded RNA genomes, which are the largest among known RNA viruses (27-31 kb). The initial step in coronavirus infection involves the binding of the viral spike protein, a 139-kDa protein, to specific receptors on the host cell's surface. The spike protein serves as the primary surface antigen for the coronavirus. When analyzing culture supernatants from SARS virus-infected cells, a 46 kDa nucleocapsid protein is prominently observed. This suggests that the nucleocapsid protein is a significant immunogen, potentially valuable for early diagnostic purposes.
Description
The SARS MERS Spike-S1 antibody specifically targets the S1 domain of the spike protein found in the MERS virus.
Formulation
The antibody is provided at a concentration of 1 mg/ml in a solution of PBS containing 0.1% NaN3.
Physical Appearance
The product is a sterile filtered liquid.
Purity
The purity of the antibody is greater than 90% as determined by SDS-PAGE gel analysis.
Applications
This antibody is suitable for use in ELISA applications.
Stability
For short-term storage, the antibody should be kept at 4°C, where it is stable for up to 2 weeks. For long-term storage, it is recommended to store the antibody at -20°C.
Purification Method

Protein A affinity purified.

Type
Mouse antibody Monoclonal.
Isotype

IgG1.

Product Science Overview

Introduction

The Mouse Anti SARS MERS Spike S1 antibody is a monoclonal antibody specifically designed to target the S1 subunit of the spike proteins found in SARS-CoV (Severe Acute Respiratory Syndrome Coronavirus) and MERS-CoV (Middle East Respiratory Syndrome Coronavirus). These spike proteins play a crucial role in the virus’s ability to infect host cells, making them a key target for therapeutic and diagnostic applications.

Spike Protein Structure

The spike protein of coronaviruses is a large type I transmembrane protein that is essential for viral entry into host cells. It consists of two subunits:

  • S1 Subunit: Contains the receptor-binding domain (RBD) responsible for recognizing and binding to the host cell receptor.
  • S2 Subunit: Facilitates the fusion of the viral and host cell membranes, allowing the viral genome to enter the host cell.
Importance of S1 Subunit

The S1 subunit is particularly important because it contains the RBD, which is the primary target for neutralizing antibodies. By binding to the RBD, these antibodies can block the virus from attaching to and entering host cells, thereby preventing infection.

Development of Mouse Anti SARS MERS Spike S1 Antibody

The development of the Mouse Anti SARS MERS Spike S1 antibody involves several key steps:

  1. Immunization: Mice are immunized with a recombinant form of the spike S1 protein to elicit an immune response.
  2. Hybridoma Technology: B cells from the immunized mice are fused with myeloma cells to create hybridomas, which are screened for the production of antibodies that specifically bind to the S1 subunit.
  3. Purification: The selected monoclonal antibodies are purified from the hybridoma culture supernatant using protein A or G affinity chromatography.
Applications

The Mouse Anti SARS MERS Spike S1 antibody has several important applications:

  • Diagnostic Testing: Used in ELISA and Western blot assays to detect the presence of SARS-CoV and MERS-CoV in clinical samples.
  • Research: Helps in studying the mechanisms of viral entry and the development of antiviral therapies.
  • Therapeutics: Potential use in passive immunization strategies to provide immediate protection against SARS-CoV and MERS-CoV infections.

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