HEK293 Cells.
Protein is >90% pure as determined SDS-PAGE.
The HEK293 derived Biotinylated recombinant protein contains the Coronavirus 2019 CoV-2 Spike Glycoprotein S1, Wuhan-Hu-1 strain, amino acids 16-685 fused to His tag & Avi-tag at C-terminal.
The 2019 novel coronavirus (2019-nCoV), a human-infecting coronavirus responsible for causing viral pneumonia, emerged in a seafood market in Wuhan, Hubei province, China, in December 2019.
Genetic analysis reveals that 2019-nCoV shares 87% of its genetic identity with the bat-derived severe acute respiratory syndrome coronavirus (SARS-CoV-2) discovered in Zhoushan, eastern China, in 2018. Despite some structural differences, the receptor-binding domain (RBD) of 2019-nCoV is similar to that of 2018 SARS-CoV, suggesting that 2019-nCoV may also bind to the human angiotensin-converting enzyme 2 (ACE2) receptor protein.
While bats are considered the likely natural reservoir of 2019-nCoV, researchers believe that an intermediary animal host, potentially one sold at the seafood market, played a role in its transmission to humans. Analysis suggests that 2019-nCoV's spike glycoprotein is a product of recombination between a bat coronavirus and an as-yet-unidentified coronavirus.
This product consists of a biotinylated recombinant protein derived from HEK293 cells. The protein comprises amino acids 16 to 685 of the Wuhan-Hu-1 strain of the Coronavirus 2019 (CoV-2) Spike Glycoprotein S1, fused with a His tag and an Avi-tag at the C-terminal end.
The CoV-2 S1 protein is supplied in a solution of 1x PBS (pH 7.4) containing 5% trehalose.
Lyophilized CoV-2 Spike S1 Glycoprotein remains stable at room temperature for up to three weeks, but for optimal preservation, it should be stored in a dry environment below -18 degrees Celsius. After reconstitution, the CoV2 Spike protein should be stored at 4 degrees Celsius for no longer than 2-7 days. For prolonged storage, freezing below -18 degrees Celsius is recommended. To ensure optimal stability during long-term storage, adding a carrier protein, such as 0.1% HSA or BSA, is advisable. Repeated cycles of freezing and thawing should be avoided.
The protein exhibits a purity greater than 90%, as determined by SDS-PAGE analysis.
HEK293 Cells.
Purified by Metal-Afinity chromatographic technique.
The Coronavirus 2019 Spike Glycoprotein-S1 (16-685 a.a.), Biotinylated Recombinant, is a crucial component in the study of SARS-CoV-2, the virus responsible for the COVID-19 pandemic. This recombinant protein is specifically designed for research purposes, aiding in the understanding of viral entry mechanisms, immune responses, and potential therapeutic targets.
The spike (S) glycoprotein of SARS-CoV-2 is a large, trimeric protein that protrudes from the viral surface. It plays a key role in the virus’s ability to infect host cells. The S protein is divided into two subunits:
The biotinylation of the recombinant S1 protein allows for easy detection and purification in various experimental setups, enhancing its utility in research.
The S glycoprotein is heavily glycosylated, with numerous N-linked and O-linked glycosylation sites. These glycans play significant roles in the protein’s function and immune evasion:
The biotinylated recombinant S1 protein is widely used in various research applications: