CoV-2-S1 (319-541), Biotin
HEK293 Cells.
Lyophilized freezed dried powder.
Protein is >90% pure as determined SDS-PAGE.
Description
The HEK293 derived Biotinylated recombinant protein contains the Coronavirus 2019 Spike Glycoprotein S1 Receptor Binding Domain [ RBD ], Wuhan-Hu-1 strain, amino acids 319-541 fused to His tag & AVI tag at C-terminal and having a molecular mass of 28.7kDa.
Product Specs
The 2019 novel coronavirus (2019-nCoV), a human-infecting coronavirus causing viral pneumonia, emerged in a Wuhan, Hubei province, China fish market in December 2019. This virus, closely related to the 2018 SARS-CoV-2 found in bats in Zhoushan, eastern China (sharing 87% identity), possesses a similar receptor-binding domain (RBD) structure despite amino acid variations. This similarity suggests that 2019-nCoV may bind to the human ACE2 receptor (angiotensin-converting enzyme 2). While bats are considered the likely reservoir, researchers speculate an intermediate animal host, possibly from the seafood market. Analysis suggests 2019-nCoV is a recombinant virus, with its spike glycoprotein originating from a bat coronavirus and an unknown coronavirus.
This product is a biotinylated recombinant protein derived from HEK293 cells. It consists of the Receptor Binding Domain (RBD) of the Coronavirus 2019 Spike Glycoprotein S1, specifically the Wuhan-Hu-1 strain, encompassing amino acids 319-541. A His tag and an AVI tag are fused to the C-terminal. The protein has a molecular mass of 28.7kDa.
The product is supplied as a lyophilized, freeze-dried powder.
The CoV-2 S1 RBD protein is lyophilized in a solution of 1x PBS at pH 7.4 containing 10% trehalose.
To reconstitute the lyophilized CoV-2 S1 protein, it is recommended to dissolve it in sterile 18M-cm H2O to a concentration of 0.2 mg/ml, ensuring it is not less than 0.1 mg/ml. This solution can be further diluted in other aqueous solutions as needed.
Lyophilized Cov-2 S1 Glycoprotein RBD remains stable at room temperature for up to 3 weeks; however, for extended storage, it is recommended to store it desiccated below -18°C. After reconstitution, the CoV2 Spike protein should be stored at 4°C for a period of 2-7 days. For long-term storage, freezing below -18°C is recommended. Adding a carrier protein (0.1% HSA or BSA) to the solution is also recommended for long-term storage. Avoid repeated freeze-thaw cycles to maintain protein integrity.
The protein purity is greater than 90% as determined by SDS-PAGE analysis.
HEK293 Cells.
Purified by Metal-Afinity chromatographic technique.
Product Science Overview
The Coronavirus 2019 (COVID-19) pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to extensive research on its structural proteins, particularly the spike (S) glycoprotein. The spike glycoprotein is crucial for the virus’s ability to infect host cells, making it a primary target for therapeutic and vaccine development.
The spike glycoprotein of SARS-CoV-2 is a transmembrane protein that forms homotrimers protruding from the viral surface. It is composed of two subunits, S1 and S2. The S1 subunit contains the receptor-binding domain (RBD), which is responsible for binding to the host cell receptor angiotensin-converting enzyme 2 (ACE2). The S2 subunit facilitates the fusion of the viral and host cell membranes .
The RBD within the S1 subunit spans amino acids 319 to 541. This domain is critical for the virus’s ability to attach to the ACE2 receptor on host cells, initiating the process of viral entry. The RBD is a major target for neutralizing antibodies, making it a focal point for vaccine and therapeutic antibody development .
Biotinylation is a process that involves attaching biotin (a vitamin) to proteins, which can then be used in various biochemical assays. The biotinylated recombinant RBD (319-541 a.a) is produced using recombinant DNA technology, where the gene encoding the RBD is inserted into an expression system, such as bacteria or mammalian cells, to produce the protein in large quantities. This biotinylated form allows for easy detection and purification in research applications .
The biotinylated recombinant RBD is widely used in research to study the interaction between the spike protein and ACE2, as well as to screen for potential inhibitors that can block this interaction. It is also used in the development of diagnostic assays and in the evaluation of immune responses to SARS-CoV-2 infection and vaccination .
