SARS Nucleocapsid (340-390), His
Protein is >90% pure as determined SDS-PAGE.
Description
The E.coli derived recombinant protein contains the Nucleocapsid C-Terminal protein 340-390 amino acids immunodominant regions fused to 6xHis tag at C-terminal.
Product Specs
The SARS-Associated Coronavirus Nucleocaspid is a key structural component of the virus, enveloped and containing three primary external antigens: membrane (M), envelope (E), and spike (S) proteins. The spike (S) glycoprotein plays a critical role in viral infection by binding to host cell receptors and facilitating viral entry. This interaction makes the S-protein a primary target for neutralizing antibodies.
This recombinant protein is derived from E. coli and comprises the C-terminal region (amino acids 340-390) of the SARS Coronavirus Nucleocapsid protein. This region, known for its immunodominant properties, is expressed with a 6xHis tag fused to its C-terminus.
The SARS Nucleocapsid protein solution is provided in a phosphate-buffered saline (PBS) solution.
To ensure stability during transport, the protein is shipped with ice packs. Upon receipt, it is recommended to store the protein at -20°C.
Analysis by SDS-PAGE confirms that the protein purity exceeds 90%.
Purified by proprietary chromatographic technique.
Immunoreactive with sera of SARS-infected individuals.
Product Science Overview
The SARS-Associated Coronavirus Nucleocapsid (340-390 a.a.) Recombinant, His Tag is a recombinant protein derived from the nucleocapsid (N) protein of the SARS-associated coronavirus. This protein segment, spanning amino acids 340 to 390, is fused with a His tag to facilitate purification and detection. The nucleocapsid protein plays a crucial role in the viral life cycle and is a key target for diagnostic and therapeutic applications.
The nucleocapsid protein is one of the four main structural proteins of coronaviruses, along with the spike (S), envelope (E), and membrane (M) proteins . It is highly immunogenic and is involved in several vital functions:
- RNA Binding: The N protein binds to the viral RNA genome, packaging it into a ribonucleoprotein complex.
- Viral Assembly: It plays a critical role in the assembly and release of new virions.
- Immune Response: Due to its high immunogenicity, the N protein is a major target for the host immune response .
The recombinant Nucleocapsid protein has several important applications:
- Diagnostics: It is used in serological assays to detect antibodies against SARS-CoV, aiding in the diagnosis of past infections.
- Vaccine Development: The immunogenic properties of the N protein make it a potential candidate for vaccine development.
- Research: It is used in various research studies to understand the structure, function, and interactions of the N protein .
