COMMD6 Human
Description
COMMD6 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
COMM Domain Containing 6 (COMMD6) is a protein encoded by the COMMD6 gene in humans. This protein is part of the COMM domain-containing family, which is known for its role in various cellular processes, including the regulation of NF-kappa-B signaling pathways . The COMM domain is a conserved region found in all members of this family, and it is crucial for their function.
COMMD6 is a small protein consisting of 108 amino acids with a molecular mass of approximately 12 kDa . The recombinant form of COMMD6 is typically produced in Escherichia coli (E. coli) and is fused to a 23 amino acid His-tag at the N-terminus to facilitate purification . The protein is non-glycosylated and is purified using proprietary chromatographic techniques to achieve a purity greater than 90% as determined by SDS-PAGE .
COMMD6 plays a significant role in the negative regulation of NF-kappa-B signaling. NF-kappa-B is a transcription factor that is involved in the expression of various genes related to immune and inflammatory responses. By down-regulating the activation of NF-kappa-B, COMMD6 helps to modulate these responses and maintain cellular homeostasis . Additionally, COMMD6 inhibits TNF-induced NFKB1 activation, further contributing to its regulatory functions .
The recombinant form of COMMD6 is produced using E. coli expression systems. The gene encoding COMMD6 is cloned into an expression vector, which is then introduced into E. coli cells. The bacteria are cultured under conditions that promote the expression of the recombinant protein. After expression, the protein is purified using chromatographic techniques, such as anion-exchange chromatography and gel-filtration chromatography .
The recombinant COMMD6 protein is typically stored in a solution containing Tris-HCl buffer, glycerol, sodium chloride, and dithiothreitol (DTT) to maintain its stability . For short-term storage, the protein can be kept at 4°C, while for long-term storage, it is recommended to store the protein at -20°C with the addition of a carrier protein to prevent degradation .
Recombinant COMMD6 is used in various research applications, including studies on NF-kappa-B signaling, protein-protein interactions, and the role of COMMD6 in cellular processes. The protein’s high purity and stability make it suitable for use in SDS-PAGE, mass spectrometry, and other biochemical assays .
