CMV Pp65, 561 a.a.

Cytomegalovirus (CMV) is a member of the Betaherpesvirinae subfamily of Herpesviridae, which also includes herpes simplex viruses types 1 and 2, varicella-zoster virus, and Epstein-Barr virus . CMV is known for its ability to remain latent in the body for long periods. It has the largest genome among the herpesviruses, ranging from 230-240 kilobase pairs . CMV is a double-stranded linear DNA virus with 162 hexagonal protein capsomeres surrounded by a lipid membrane .

The CMV Pp65 (UL83) protein is one of the most immunodominant antigens of the virus. It plays a crucial role in the immune response against CMV infections. The recombinant form of this protein, consisting of 561 amino acids, is derived from Escherichia coli (E. coli) and has a molecular weight of approximately 62.8 kDa .

The recombinant CMV Pp65 protein is produced in E. coli and purified using proprietary chromatographic techniques . The protein is formulated in a buffer containing 25mM Tris-HCl (pH 8), 8M urea, and 5mM β-mercaptoethanol (βMe) . It is highly pure, with a purity level greater than 95% as determined by SDS-PAGE .

The CMV Pp65 protein is stable at 4°C for up to one week but should be stored below -18°C for long-term storage to prevent freeze-thaw cycles .

The CMV Pp65 antigen is suitable for use in enzyme-linked immunosorbent assays (ELISA) and Western blots. It is an excellent antigen for detecting CMV with minimal specificity problems . The protein is immunoreactive with sera from CMV-infected individuals, making it a valuable tool for diagnostic and research purposes .