Clusterin Canine, HEK
Clusterin Canine Recombinant, HEK
Cat. No.
BT12632
Source
Human Embryonic Kidney 293 Cells.
Synonyms
CLI, AAG4, KUB1, SGP2, SGP-2, SP-40, TRPM2, MGC24903, Clusterin, Glycoprotein 80, Gp80, CLU.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95% as determined by SDS PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Clusterin Canine Recombinant produced in HEK293 cells is a glycosylated, Polypeptide chain containing 436 amino acids and having a molecular mass of 50.72 kDa. The protein is fused with 13 amino acid Flag tag at N-Terminus.
The Apolipoprotein-J Canine is purified by proprietary chromatographic techniques.
The Apolipoprotein-J Canine is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Studies indicate that androgen treatment increases Clusterin mRNA and protein levels. Clusterin's interaction with the LDL-Receptor appears significant in membranous glomerulonephritis development. In CaP, Clusterin expression decreases compared to benign controls. Additionally, Clusterin has been implicated in cellular senescence, tumorigenesis, and the photo-oxidative cell death pathway. Furthermore, it serves as a functional tumor marker for diagnosing pediatric large cell lymphoma. Clusterin is activated in acidic environments and can inhibit NF-kappaB signaling by stabilizing IkappaBs, which can limit tumor cell movement. Interestingly, the N-terminal deletion of Clusterin seems crucial for its altered biogenesis in esophageal squamous cell carcinoma.
Description
Produced in HEK293 cells, Canine Clusterin is a glycosylated polypeptide chain comprising 436 amino acids. With a molecular weight of 50.72 kDa, this recombinant protein features a 13 amino acid Flag tag at its N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
White, lyophilized powder.
Formulation
The Canine Clusterin undergoes filtration (0.4µm) and lyophilization from a solution of 0.5mg/ml containing 20mM Tris buffer and 20mM NaCl at a pH of 7.5.
Solubility
To create a working stock solution, add deionized water to achieve a concentration of roughly 0.5mg/ml. Ensure complete dissolution of the lyophilized pellet. Please note that this product is not sterile. Prior to use in cell culture, filter the solution through a sterile filter.
Stability
Lyophilized protein should be stored at -20°C. After reconstitution, aliquot the product to minimize freeze-thaw cycles. Reconstituted protein remains stable at 4°C for a limited period; no changes are observed for up to two weeks at this temperature.
Purity
Purity exceeds 95% as determined by SDS-PAGE analysis.
Synonyms
CLI, AAG4, KUB1, SGP2, SGP-2, SP-40, TRPM2, MGC24903, Clusterin, Glycoprotein 80, Gp80, CLU.
Source
Human Embryonic Kidney 293 Cells.
Amino Acid Sequence
PGDYKDDDDK PAGDQAVSDT ELQEMSTEGS KYINKEIKNA LKGVKQIKTL IEQTNEERKS LLSNLEEAKK KKEDALNDTK DSETKLKASQ GVCNDTMMAL WEECKPCLKQ TCMKFYARVC RSGSGLVGHQ LEEFLNQSSP FYFWMNGDRI DSLLENDRQQ THALDVMQDS FNRASSIMDE LFQDRFFTRE PQDTYHYSPF SLFQRRPFFN PKFRIARNII PFPRFQPLNF HDMFQPFFDM IHQAQQAMDV NLHRIPYHFP IEFPEEDNRT VCKEIRHNST GCLKMKDQCE KCQEILSVDC SSNNPAQVQL RQELSNSLQI AEKFTKLYDE LLQSYQEKMF NTSSLLKQLN EQFSWVSQLA NLTQSEDPFY LQVTTVGSQT SDSNVPVGFT KVVVKLFDSD PITVMIPEAV SRNNPKFMET VAEKALQEYRQKHREE.
Product Science Overview
Production and Structure
Clusterin Canine Recombinant is produced in HEK293 cells (Human Embryonic Kidney 293 cells), which are widely used in biotechnology for the production of recombinant proteins. The recombinant protein is a single, glycosylated polypeptide chain containing 436 amino acids and has a molecular mass of 50.72 kDa . The protein is fused with a 13 amino acid Flag tag at the N-terminus, which aids in its purification and detection .
Functions and Applications
Clusterin is involved in several critical biological functions:
- Lipid Transport: It assists in the transport of lipids in the bloodstream.
- Cell Apoptosis: It plays a role in the regulation of programmed cell death.
- Tissue Remodeling: It is involved in the remodeling of tissues during development and in response to injury.
In research and biotechnology, recombinant Clusterin is used to study these functions and to develop therapeutic strategies for diseases where Clusterin is implicated, such as neurodegenerative diseases, cancer, and cardiovascular diseases.
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