Clusterin Antibody
Description
Product Specs
Product Science Overview
Clusterin is composed of two 40 kDa chains, alpha and beta, which are covalently linked by disulfide bonds . The protein is known for its chaperone-like activity, helping to prevent the aggregation of misfolded proteins. This function is particularly important in the context of neurodegenerative diseases such as Alzheimer’s disease, where clusterin inhibits the formation of amyloid plaques .
In addition to its chaperone activity, clusterin is involved in the regulation of the complement system. It is a component of the SC5b-9 complex, which prevents the binding of the C5b-C7 complex to the target cell membrane, thereby inhibiting the complement cascade . This regulatory role is crucial in protecting cells from immune-mediated damage.
Clusterin is overexpressed in various cancers, including prostate, breast, and squamous cell carcinomas . Its expression is associated with tumor progression and resistance to chemotherapy. By inhibiting apoptotic signaling pathways, clusterin helps cancer cells survive and proliferate. Consequently, targeting clusterin has been explored as a potential therapeutic strategy in cancer treatment.
The Mouse Anti-Human Clusterin Antibody is a monoclonal antibody that specifically binds to human clusterin. This antibody is commonly used in research to study the expression and function of clusterin in various biological contexts. It is particularly useful in immunohistochemistry (IHC) and Western blotting applications .
In IHC, the Mouse Anti-Human Clusterin Antibody can be used to stain formalin-fixed, paraffin-embedded tissue sections. For example, it has been used to detect clusterin expression in human tonsil tissue, where it stains dendritic cells in the follicle and the basement membrane of epithelial cells . The antibody is typically used in combination with secondary antibodies and detection systems to visualize the staining.
In Western blotting, the antibody can be used to detect clusterin in cell lysates or tissue extracts. It is important to use appropriate controls and optimize the antibody concentration to obtain specific and reliable results.
