CLTB Human
Description
CLTB is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
EEAFVKESKE ETPGTEWEKV AQLCDFNPKS SKQCKDVSRL RSVLMSLKQT PLSR.
Product Science Overview
Clathrin is a large, soluble protein composed of three heavy chains and three light chains, forming a triskelion shape . This triskelion structure is the fundamental unit that polymerizes to form a clathrin-coated vesicle. The light chains, including CLTB, are integral to the stability and function of these vesicles .
CLTB specifically is a member of the clathrin light chain family and is involved in the structural integrity of the lattice-type cytoplasmic face of coated pits and vesicles . These pits and vesicles capture specific macromolecules during receptor-mediated endocytosis, a process critical for cellular communication and nutrient uptake .
Recombinant human CLTB is produced using Escherichia coli (E. coli) expression systems. This recombinant protein typically includes a His-tag at the N-terminus and corresponds to the amino acids 1-211 of the human CLTB sequence . The recombinant form is used in various research applications to study the protein’s function and interactions within the cell.
The recombinant CLTB protein is purified to a high degree, often exceeding 85% purity, and is validated using techniques such as SDS-PAGE and mass spectrometry . It is stored in specific buffer conditions to maintain its stability and functionality over time .
Recombinant CLTB is widely used in research to understand the mechanisms of clathrin-mediated endocytosis and its role in cellular physiology. By studying the interactions and functions of CLTB, researchers can gain insights into various cellular processes, including:
- Internalization of Growth Factors and Receptors: CLTB is involved in the internalization of growth factors and their receptors, which is crucial for cell signaling and growth regulation .
- Pathogen Entry: Some pathogens exploit clathrin-mediated endocytosis to enter host cells. Understanding CLTB’s role in this process can help develop strategies to prevent infections .
- Synaptic Transmission: In neurons, clathrin-mediated endocytosis is essential for the recycling of synaptic vesicles, which is vital for neurotransmission .
