CLTA Human

Clathrin is composed of three heavy chains and three light chains, forming a triskelion structure. The light chains, including Clathrin Light Chain A (CLTA), are crucial for the regulation of clathrin assembly and disassembly. CLTA is a non-glycosylated polypeptide chain that contains 242 amino acids and has a molecular mass of approximately 26.2 kDa . The light chains are involved in stabilizing the clathrin triskelion and modulating its interaction with other proteins.

Human Clathrin Light Chain A (CLTA) can be produced recombinantly in Escherichia coli (E. coli) expression systems. The recombinant protein is typically fused to a His-tag at the N-terminus to facilitate purification. The purified recombinant CLTA is used in various research applications, including blocking assays and control experiments .

Recombinant CLTA is widely used in research to study clathrin-mediated endocytosis and intracellular trafficking. It serves as a valuable tool for investigating the molecular mechanisms underlying these processes. The recombinant protein can be used in various assays, such as SDS-PAGE and mass spectrometry (MS), to analyze its purity and functionality .

Clathrin-mediated endocytosis is essential for maintaining cellular homeostasis and regulating signal transduction pathways. The clathrin coat facilitates the formation of vesicles that transport cargo from the plasma membrane to intracellular compartments, such as endosomes and lysosomes. This process is critical for the uptake of nutrients, receptor internalization, and the regulation of cell surface receptor levels.