CLMP Human, SF9
Sf9, Baculovirus cells.
CXADR like membrane protein, ACAM, ASAM, CSBM, CSBS, Adipocyte adhesion molecule, Coxsackie- and adenovirus receptor-like membrane protein, CAR-like membrane protein.
Greater than 95.0% as determined by SDS-PAGE.
Description
CLMP produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 226 amino acids (19-235a.a.) and having a molecular mass of 25.6kDa. (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
CLMP is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Belonging to the CTX family within the immunoglobulin superfamily, CXADR Like Membrane Protein (CLMP) is a type 1 transmembrane peptide. It is also known as CXADR-like membrane protein. CLMP is a part of junction complexes across endothelial and epithelial cells and may be involved in cell adhesion. It is found in various tissues, including the placenta, small intestine, skeletal muscle, colon, lungs, and heart. Research suggests that CLMP may play a role in obesity development by influencing adipocyte differentiation.
Produced in Sf9 Baculovirus cells, CLMP is a single, glycosylated polypeptide chain with a molecular weight of 25.6 kDa. It consists of 226 amino acids (19-235a.a.). On SDS-PAGE, the molecular size appears to be approximately 28-40 kDa. CLMP is expressed with a 9 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
The CLMP protein solution has a concentration of 0.5 mg/ml and contains phosphate buffered saline (pH 7.4), 1 mM DTT, and 20% glycerol.
The purity of CLMP is greater than 95.0% as determined by SDS-PAGE analysis.
CXADR like membrane protein, ACAM, ASAM, CSBM, CSBS, Adipocyte adhesion molecule, Coxsackie- and adenovirus receptor-like membrane protein, CAR-like membrane protein.
Sf9, Baculovirus cells.
ADPTHTEIKR VAEEKVTLPC HHQLGLPEKD TLDIEWLLTD NEGNQKVVIT YSSRHVYNNL TEEQKGRVAF ASNFLAGDAS LQIEPLKPSD EGRYTCKVKN SGRYVWSHVI LKVLVRPSKP KCELEGELTE GSDLTLQCES SSGTEPIVYY WQRIREKEGE DERLPPKSRI DYNHPGRVLL
QNLTMSYSGL YQCTAGNEAG KESCVVRVTV QYVQSIGMVA HHHHHH.
Product Science Overview
CXADR-Like Membrane Protein, also known as Coxsackievirus and Adenovirus Receptor-Like Membrane Protein (CLMP), is a transmembrane protein that plays a crucial role in cell adhesion and signaling. This protein is particularly significant in cardiac and testicular functions due to its homophilic and heterophilic interactions .
The CXADR-Like Membrane Protein consists of several key regions:
- Extracellular Domain (ECD): This domain contains a signal sequence and two immunoglobulin (Ig) loops, the V and C2 domains, which are essential for its function in cell adhesion .
- Transmembrane Region: This single membrane-spanning segment anchors the protein to the cell membrane .
- Intracellular Domain (ICD): This domain is involved in intracellular signaling and interactions with other proteins .
The recombinant form of CXADR-Like Membrane Protein is typically produced in Sf9 Baculovirus cells. This method involves the expression of the protein in insect cells, which allows for proper folding and post-translational modifications. The protein is then purified using various chromatographic techniques to ensure high purity and functionality .
CXADR-Like Membrane Protein is involved in several critical biological processes:
- Cell Adhesion: It mediates cell-cell adhesion through its extracellular Ig domains, which interact with similar domains on adjacent cells .
- Signaling: The intracellular domain of the protein interacts with various signaling molecules, playing a role in processes such as spermatogenesis and cardiac function .
- Protection Against Injury: Recent studies have shown that CXADR-Like Membrane Protein can protect against heart injury by preventing excessive pyroptosis, a form of programmed cell death, after myocardial infarction .
CXADR-Like Membrane Protein participates in several biochemical interactions:
- Disulfide Bond Formation: The protein contains cysteine residues in its Ig domains that form intrachain disulfide bonds, stabilizing its structure .
- Protein-Protein Interactions: It interacts with other proteins at cell junctions, contributing to the integrity and function of tissues such as the heart and testes .
