CLIC4 Human

Chloride Intracellular Channel 4 Human Recombinant
Cat. No.
BT7770
Source
Escherichia Coli.
Synonyms
Chloride intracellular channel protein 4, Intracellular chloride ion channel protein p64H1, CLIC4, H1, huH1, p64H1, CLIC4L, MTCLIC, FLJ38640, DKFZp566G223.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CLIC4 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 273 amino acids (1-253 a.a.) and having a molecular mass of 30.9kDa. The CLIC4 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Chloride intracellular channel 4 (CLIC4) is a member of the p64 family found in various tissues. It displays an intracellular vesicular pattern within Panc-1 cells (pancreatic cancer cells). This 253-amino acid protein is a single-pass membrane protein located in both the nucleus and cytoplasm, containing one GST C-terminal domain. Functioning as a monomer, CLIC4 forms selective ion channels in target proteins, facilitating the transport of chloride and other ions. CLIC4 is implicated in apoptosis and can translocate to the nucleus when under stress.
Description
Recombinant human CLIC4 is fused with a 20 amino acid His tag at its N-terminus and produced in E. coli. It exists as a single, non-glycosylated polypeptide chain consisting of 273 amino acids (1-253 a.a.) with a molecular weight of 30.9kDa. Purification of CLIC4 is achieved through proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution.
Formulation
The CLIC4 solution (0.5 mg/ml) is supplied in a buffer of 20mM Tris-HCl (pH 8.0), 0.1M NaCl, 1mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For longer storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 95.0% by SDS-PAGE analysis.
Synonyms
Chloride intracellular channel protein 4, Intracellular chloride ion channel protein p64H1, CLIC4, H1, huH1, p64H1, CLIC4L, MTCLIC, FLJ38640, DKFZp566G223.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MALSMPLNGL KEEDKEPLIE LFVKAGSDGE SIGNCPFSQR LFMILWLKGV VFSVTTVDLK RKPADLQNLA PGTHPPFITF NSEVKTDVNK IEEFLEEVLC PPKYLKLSPK HPESNTAGMD IFAKFSAYIK NSRPEANEAL ERGLLKTLQK LDEYLNSPLP DEIDENSMED IKFSTRKFLD GNEMTLADCN LLPKLHIVKV VAKKYRNFDI PKEMTGIWRY LTNAYSRDEF TNTCPSDKEV EIAYSDVAKR LTK.

Product Science Overview

Introduction

Chloride Intracellular Channel 4 (CLIC4), also known as p64H1, is a member of the chloride intracellular channel (CLIC) family. These proteins are unique in that they can exist both in soluble and membrane-bound forms, allowing them to participate in a variety of cellular processes. CLIC4 is encoded by the CLIC4 gene and is expressed in many tissues throughout the body .

Structure and Function

CLIC4 is a protein consisting of 253 amino acids . It has a single transmembrane domain, which allows it to insert into cellular membranes and form ion channels. This insertion is redox-regulated, meaning it occurs under oxidizing conditions . In its soluble state, CLIC4 can catalyze thiol-disulfide exchange reactions, similar to glutaredoxins, using reduced glutathione as an electron donor .

Cellular Roles

CLIC4 plays several critical roles within cells:

  • Ion Transport: It forms voltage-dependent, multi-ion conductive channels that facilitate the transport of chloride and other ions across intracellular membranes .
  • Angiogenesis: CLIC4 is involved in the formation of new blood vessels, a process crucial for wound healing and tumor growth .
  • Cell Proliferation and Morphogenesis: It promotes endothelial cell proliferation and regulates endothelial morphogenesis, contributing to the maintenance of apical-basolateral membrane polarity during mitosis and cytokinesis .
  • Cardioprotection: CLIC4 has been implicated in protecting the heart from ischemia-reperfusion injury, a type of damage caused by the restoration of blood supply after a period of ischemia or lack of oxygen .
Clinical Significance

CLIC4 is associated with various diseases and conditions:

  • Cancer: Abnormal expression of CLIC4 has been linked to several types of cancer, including pancreatic cancer .
  • Pulmonary Hypertension: CLIC4 is implicated in the pathogenesis of pulmonary hypertension, a condition characterized by high blood pressure in the lungs .
  • Cardiovascular Diseases: Its role in cardioprotection makes it a potential target for therapeutic interventions in cardiovascular diseases .
Binding Partners

CLIC4 interacts with several other proteins, including dynamin I, α-tubulin, β-actin, creatine kinase, and two 14-3-3 isoforms . These interactions suggest that CLIC4 is involved in various cellular processes, such as cytoskeletal organization and membrane trafficking.

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